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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

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Autor(es):
Pinho, Laura Costa [1, 2] ; Garbieri, Thais Francini [3, 1] ; Grenho, Liliana [1, 4] ; Alves, Marta M. [5] ; Sousa Gomes, Pedro [1, 4] ; Santos, Carlos Ferreira [3] ; Fernandes, Maria Helena [1, 4] ; Santos, Catarina [5, 6] ; Colaco, Bruno [4, 2, 7]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Porto, Fac Med Dent, Lab Bone Metab & Regenerat, P-4200393 Porto - Portugal
[2] Univ Tras Os Montes & Alto Douro, Dept Anim Sci, P-5000801 Vila Real - Portugal
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil
[4] Univ Porto, LAQV REQUIMTE, P-4160007 Porto - Portugal
[5] Univ Lisbon, Inst Super Tecn, Ctr Quim Estrutural, P-1049001 Lisbon - Portugal
[6] Inst Politecn Setubal, CDP2T, EST Setubal, Campus IPS, P-2910761 Setubal - Portugal
[7] Univ Tras Os Montes & Alto Douro, CECAV Anim & Vet Res Ctr UTAD, P-5000801 Vila Real - Portugal
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: MATERIALS; v. 14, n. 15 AUG 2021.
Citações Web of Science: 0
Resumo

Considering the role of magnesium in bone metabolism and the increasing relevance of plant-mediated green-synthesis, this work compares the bone cytocompatibility of magnesium hydroxide nanoparticles (NPs) produced by using pure water, Mg(OH)(2), or a rosehip (RH) aqueous extract, Mg(OH)(2)RH. The NPs were evaluated for dose- and time-dependent effects on human osteoblastic and osteoclastic response, due to the direct involvement of the two cell types in bone metabolism. Mg(OH)(2) NPs presented nanoplatelet-like morphology (mean diameter similar to 90 nm) and a crystalline structure (XRD analysis); the RH-mediated synthesis yielded smaller rounded particles (mean diameter <10 nm) with decreased crystallinity. On the ATR-FTIR spectra, both NPs presented the characteristic Mg-OH peaks; Mg(OH)(2)RH exhibited additional vibration bands associated with the presence of phytochemicals. On osteoblastic cells, NPs did not affect cell growth and morphology but significantly increased alkaline phosphatase (ALP) activity; on osteoclastic cells, particles had little effect in protein content, tartrate-resistant acid phosphatase (TRAP) activity, percentage of multinucleated cells, and cell area. However, compared with Mg(OH)(2), Mg(OH)(2)RH increased osteoblastic differentiation by inducing ALP activity and promoting the expression of Runx2, SP7, Col1a1, and ALP, and had a negative effect on the expression of the osteoclastic genes NFATC1, CA2, and CTSK. These observations suggest the potential usefulness of Mg(OH)(2)RH NPs in bone regeneration. (AU)

Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
Beneficiário:Carlos Ferreira dos Santos
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 17/19227-6 - Investigação da modulação da resposta inflamatória em células orais e imunológicas humanas por Ang II e Ang 1-7
Beneficiário:Thais Francini Garbieri
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 18/23934-2 - Papel da angiotensina ii e da angiotensina 1-7 no metabolismo de células ósseas humanas
Beneficiário:Thais Francini Garbieri
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado