Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

Author(s)

Pinho, Laura Costa ^{[1, 2]} ; Garbieri, Thais Francini ^{[3, 1]} ; Grenho, Liliana ^{[1, 4]} ; Alves, Marta M. ^[5] ; Sousa Gomes, Pedro ^{[1, 4]} ; Santos, Carlos Ferreira ^[3] ; Fernandes, Maria Helena ^{[1, 4]} ; Santos, Catarina ^{[5, 6]} ; Colaco, Bruno ^{[4, 2, 7]}

Affiliation

^[1] Univ Porto, Fac Med Dent, Lab Bone Metab & Regenerat, P-4200393 Porto - Portugal

^[2] Univ Tras Os Montes & Alto Douro, Dept Anim Sci, P-5000801 Vila Real - Portugal

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[4] Univ Porto, LAQV REQUIMTE, P-4160007 Porto - Portugal

^[5] Univ Lisbon, Inst Super Tecn, Ctr Quim Estrutural, P-1049001 Lisbon - Portugal

^[6] Inst Politecn Setubal, CDP2T, EST Setubal, Campus IPS, P-2910761 Setubal - Portugal

^[7] Univ Tras Os Montes & Alto Douro, CECAV Anim & Vet Res Ctr UTAD, P-5000801 Vila Real - Portugal

• Document type: Journal article
• Source: MATERIALS; v. 14, n. 15 AUG 2021.
• Web of Science Citations: 0

Abstract

Considering the role of magnesium in bone metabolism and the increasing relevance of plant-mediated green-synthesis, this work compares the bone cytocompatibility of magnesium hydroxide nanoparticles (NPs) produced by using pure water, Mg(OH)(2), or a rosehip (RH) aqueous extract, Mg(OH)(2)RH. The NPs were evaluated for dose- and time-dependent effects on human osteoblastic and osteoclastic response, due to the direct involvement of the two cell types in bone metabolism. Mg(OH)(2) NPs presented nanoplatelet-like morphology (mean diameter similar to 90 nm) and a crystalline structure (XRD analysis); the RH-mediated synthesis yielded smaller rounded particles (mean diameter <10 nm) with decreased crystallinity. On the ATR-FTIR spectra, both NPs presented the characteristic Mg-OH peaks; Mg(OH)(2)RH exhibited additional vibration bands associated with the presence of phytochemicals. On osteoblastic cells, NPs did not affect cell growth and morphology but significantly increased alkaline phosphatase (ALP) activity; on osteoclastic cells, particles had little effect in protein content, tartrate-resistant acid phosphatase (TRAP) activity, percentage of multinucleated cells, and cell area. However, compared with Mg(OH)(2), Mg(OH)(2)RH increased osteoblastic differentiation by inducing ALP activity and promoting the expression of Runx2, SP7, Col1a1, and ALP, and had a negative effect on the expression of the osteoclastic genes NFATC1, CA2, and CTSK. These observations suggest the potential usefulness of Mg(OH)(2)RH NPs in bone regeneration. (AU)

• FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
• Grantee: Carlos Ferreira dos Santos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/19227-6 - Investigation of the modulation of the inflammatory response in human oral and immunological cells by Ang II and Ang 1-7
• Grantee: Thais Francini Garbieri
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/23934-2 - Role of angiotensin ii and angiotensin 1-7 on the metabolism of human bone cells
• Grantee: Thais Francini Garbieri
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate