Free and Substrate-Immobilised Lipases fromFusarium verticillioidesP24 as a Biocatalyst for Hydrolysis and Transesterification Reactions

Autor(es)

Borges, Janaina Pires ^[1] ; Quilles Junior, Jose Carlos ^[2] ; Ohe, Thiago Hideyuki Kobe ^[2] ; Ferrarezi, Ana Lucia ^[3] ; Nunes, Christiane da Costa Carreira ^[3] ; Boscolo, Mauricio ^[2] ; Gomes, Eleni ^[3] ; Bocchini, Daniela Alonso ^[1] ; da Silva, Roberto ^[2]

Afiliação do(s) autor(es)

^[1] UNESP, IQ, Dept Biochem & Chem Technol, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

^[2] UNESP, IBILCE, Dept Chem & Environm Sci, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] UNESP, IBILCE, Dept Biol, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 193, n. 1, p. 33-51, JAN 2021.
• Citações Web of Science: 0

Resumo

Fungal enzymes are widely used in technological processes and have some interesting features to be applied in a variety of biosynthetic courses. Here, free and substrate-immobilised lipases fromFusarium verticillioidesP24 were obtained by solid-state fermentation using wheat bran as substrate and fungal carrier. Based on their hydrolytic and transesterification activities, the lipases were characterised as pH-dependent in both reactions, with higher substrate conversion in an alkaline environment. Thermally, the lipases performed well from 30 to 45 degrees C, being more stable in mild conditions. Organic solvents significantly influenced the lipase selectivity using different vegetable oils as fatty acid source. Omega(omega)-3 production inn-hexane achieved 45% using canola oil, against approximate to 18% in cyclohexane. However, omega-6 production was preferably produced for both solvents using linseed oil with significant alterations in the yield (approximate to 79% and 49% forn-hexane and cyclohexane, respectively). Moreover, the greatest enzyme selectivity for omega-6 led us to suppose a lipase preference for the Sn1 position of the triacylglycerol. Lastly, a transesterification reaction was performed, achieving 90% of ester conversion in 72 h. This study reports the characterisation and use of free and substrate-immobilised lipases fromFusarium verticillioidesP24 as an economic and efficient method for the first time. (AU)

• Processo FAPESP: 08/58077-0 - Produção de biodiesel etílico com uso de lipases intra e extracelulares de fungos termofílicos com enzimas livres e imobilizadas em diferentes suportes
• Beneficiário: Roberto da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 10/03555-5 - Produção de lipases por fungos termofílicos e mesofílicos e uso na produção de biodiesel etílico
• Beneficiário: Janaina Pires Borges
• Modalidade de apoio: Bolsas no Brasil - Mestrado