GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri

Autor(es)

Picchi, Simone Cristina ^[1] ; de Souza e Silva, Mariana ^[1] ; Saldanha, Luiz Leonardo ^[2] ; Ferreira, Henrique ^[2] ; Takita, Marco Aurelio ^[1] ; Caldana, Camila ^{[3, 4]} ; de Souza, Alessandra Alves ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron Campinas, Ctr Citricultura Sylvio Moreira, BR-13490970 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Sao Paulo - Brazil

^[3] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, BR-13083100 Campinas, SP - Brazil

^[4] Max Planck Inst Mol Pflanzenphysiol, Wissenschaftspk Golm, Muhlenberg 1, D-14476 Potsdam - Germany

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 JUL 30 2021.
• Citações Web of Science: 0

Resumo

N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC-MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation. (AU)

• Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
• Beneficiário: Marcos Antonio Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/01395-9 - N-acetil-cisteina (NAC): uma molécula com potencial antimicrobiano para o manejo do cancro cítrico.
• Beneficiário: Simone Cristina Picchi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/10957-0 - Interação Xylella fastidiosa-inseto vetor-planta hospedeira e abordagens para o controle da clorose variegada dos citros e cancro cítrico
• Beneficiário: Alessandra Alves de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/10734-5 - Análises de metaboloma de Citrus sinensis e do fitopatógeno Xanthomonas citri sub. citri
• Beneficiário: Luiz Leonardo Saldanha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado