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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri

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Author(s):
Picchi, Simone Cristina [1] ; de Souza e Silva, Mariana [1] ; Saldanha, Luiz Leonardo [2] ; Ferreira, Henrique [2] ; Takita, Marco Aurelio [1] ; Caldana, Camila [3, 4] ; de Souza, Alessandra Alves [1]
Total Authors: 7
Affiliation:
[1] Inst Agron Campinas, Ctr Citricultura Sylvio Moreira, BR-13490970 Sao Paulo - Brazil
[2] Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Sao Paulo - Brazil
[3] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, BR-13083100 Campinas, SP - Brazil
[4] Max Planck Inst Mol Pflanzenphysiol, Wissenschaftspk Golm, Muhlenberg 1, D-14476 Potsdam - Germany
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 11, n. 1 JUL 30 2021.
Web of Science Citations: 0
Abstract

N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC-MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation. (AU)

FAPESP's process: 14/50880-0 - INCT 2014: comparative and functional genomics and citrus-assisted breeding
Grantee:Marcos Antonio Machado
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/01395-9 - N-acetyl-cysteine (NAC): a molecule with antimicrobial potential for management of citrus canker.
Grantee:Simone Cristina Picchi
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/10957-0 - Xylella fastidiosa-vector-host plant interaction and approaches for citrus variegated chlorosis and citrus canker control
Grantee:Alessandra Alves de Souza
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/10734-5 - Metabolic profiles of Xanthomonas citri sub. citri, and the plant-host response upon infection with the phytopathogen
Grantee:Luiz Leonardo Saldanha
Support Opportunities: Scholarships in Brazil - Post-Doctoral