A Family of Nonribosomal Peptides Modulate Collective Behavior in Pseudovibrio Bacteria Isolated from Marine Sponges{*}{*}

Autor(es):Mostrar menos -

Ioca, Laura P. ^{[1, 2, 3]} ; Dai, Yitao ^[3] ; Kunakom, Sylvia ^{[2, 3]} ; Diaz-Espinosa, Jennifer ^{[2, 3]} ; Krunic, Aleksej ^[2] ; Crnkovic, Camila M. ^{[2, 3]} ; Orjala, Jimmy ^{[2, 3]} ; Sanchez, Laura M. ^[2] ; Ferreira, Antonio G. ^[4] ; Berlinck, Roberto G. S. ^[1] ; Eustaquio, Alessandra S. ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Illinois, Coll Pharm, Dept Pharmaceut Sci, Chicago, IL 60607 - USA

^[3] Univ Illinois, Coll Pharm, Ctr Biomol Sci, Chicago, IL 60607 - USA

^[4] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION; v. 60, n. 29, p. 15891-15898, JUL 12 2021.
• Citações Web of Science: 0

Resumo

Although swarming motility and biofilms are opposed collective behaviors, both contribute to bacterial survival and host colonization. Pseudovibrio bacteria have attracted attention because they are part of the microbiome of healthy marine sponges. Two-thirds of Pseudovibrio genomes contain a member of a nonribosomal peptide synthetase-polyketide synthase gene cluster family, which is also found sporadically in Pseudomonas pathogens of insects and plants. After developing reverse genetics for Pseudovibrio, we isolated heptapeptides with an ureido linkage and related nonadepsipeptides we termed pseudovibriamides A and B, respectively. A combination of genetics and imaging mass spectrometry experiments showed heptapetides were excreted, promoting motility and reducing biofilm formation. In contrast to lipopeptides widely known to affect motility/biofilms, pseudovibriamides are not surfactants. Our results expand current knowledge on metabolites mediating bacterial collective behavior. (AU)

• Processo FAPESP: 15/01017-0 - EMU concedido no âmbito do projeto aprovado 2013/50228-8, nome do equipamento: uPLC-HRMS (cromatógrafo líquido de ultra resolução acoplado com espectrômetro de massas de alta resolução)
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 16/05133-7 - Estudo do metabolismo de micro-organismos do ambiente marinho: perfil químico e biossíntese
• Beneficiário: Laura Pavan Ióca
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/10742-8 - A função de um peptídeo não-ribossimal para promover a motilidade de Pseudovibrio brasiliensis Ab134 e bactérias relacionadas
• Beneficiário: Laura Pavan Ióca
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado