A Family of Nonribosomal Peptides Modulate Collective Behavior in Pseudovibrio Bacteria Isolated from Marine Sponges{*}{*}

Author(s):Show less -

Ioca, Laura P. ^{[1, 2, 3]} ; Dai, Yitao ^[3] ; Kunakom, Sylvia ^{[2, 3]} ; Diaz-Espinosa, Jennifer ^{[2, 3]} ; Krunic, Aleksej ^[2] ; Crnkovic, Camila M. ^{[2, 3]} ; Orjala, Jimmy ^{[2, 3]} ; Sanchez, Laura M. ^[2] ; Ferreira, Antonio G. ^[4] ; Berlinck, Roberto G. S. ^[1] ; Eustaquio, Alessandra S. ^{[2, 3]}

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Illinois, Coll Pharm, Dept Pharmaceut Sci, Chicago, IL 60607 - USA

^[3] Univ Illinois, Coll Pharm, Ctr Biomol Sci, Chicago, IL 60607 - USA

^[4] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION; v. 60, n. 29, p. 15891-15898, JUL 12 2021.
• Web of Science Citations: 0

Abstract

Although swarming motility and biofilms are opposed collective behaviors, both contribute to bacterial survival and host colonization. Pseudovibrio bacteria have attracted attention because they are part of the microbiome of healthy marine sponges. Two-thirds of Pseudovibrio genomes contain a member of a nonribosomal peptide synthetase-polyketide synthase gene cluster family, which is also found sporadically in Pseudomonas pathogens of insects and plants. After developing reverse genetics for Pseudovibrio, we isolated heptapeptides with an ureido linkage and related nonadepsipeptides we termed pseudovibriamides A and B, respectively. A combination of genetics and imaging mass spectrometry experiments showed heptapetides were excreted, promoting motility and reducing biofilm formation. In contrast to lipopeptides widely known to affect motility/biofilms, pseudovibriamides are not surfactants. Our results expand current knowledge on metabolites mediating bacterial collective behavior. (AU)

• FAPESP's process: 15/01017-0 - Multi-use instrument approved and awarded in the project 2013/50228-8, name of instrument: ultra-performance liquid chromatograph coupled with a high resolution mass spectrometer
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 16/05133-7 - Study of marine-derived microrganisms metabolism: chemical profile and biosynthesis
• Grantee: Laura Pavan Ióca
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/10742-8 - The role of a nonribosomal peptide in promoting swarming in Pseudovibrio brasiliensis Ab134 and related bacteria
• Grantee: Laura Pavan Ióca
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate