Neonatal anoxia increases nociceptive response in rats: Sex differences and lumbar spinal cord and insula alterations

Autor(es)

Helou, Ammir Yacoub ^[1] ; Martins, Daniel Oliveira ^[2] ; Arruda, Bruna Petrucelli ^[1] ; de Souza, Matheus Cerussi ^[1] ; Cruz-Ochoa, Natalia Andrea ^[1] ; Nogueira, Maria Ines ^[1] ; Chacur, Marucia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Neurosci Lab, Ave Prof Lineu Prestes 2415, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Funct Neuroanat Pain, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE; v. 81, n. 8 AUG 2021.
• Citações Web of Science: 0

Resumo

Neonatal anoxia is a well-known world health problem that results in neurodevelopmental deficits, such as sensory alterations that are observed in patients with cerebral palsy and autism disorder, for which oxygen deprivation is a risk factor. Nociceptive response, as part of the sensory system, has been reported as altered in these patients. To determine whether neonatal oxygen deprivation alters nociceptive sensitivity and promotes medium- and long-term inflammatory feedback in the central nervous system, Wistar rats of around 30 h old were submitted to anoxia (100% nitrogen flux for 25 min) and evaluated on PND23 (postpartum day) and PND90. The nociceptive response was assessed by mechanical, thermal, and tactile tests in the early postnatal and adulthood periods. The lumbar spinal cord (SC, L4-L6) motor neurons (MNs) and the posterior insular cortex neurons were counted and compared with their respective controls after anoxia. In addition, we evaluated the possible effect of anoxia on the expression of astrocytes in the SC at adulthood. The results showed increased nociceptive responses in both males and females submitted to anoxia, although these responses were different according to the nociceptive stimulus. A decrease in MNs in adult anoxiated females and an upregulation of GFAP expression in the SC were observed. In the insular cortex, a decrease in the number of cells of anoxiated males was observed in the neonatal period. Our findings suggest that oxygen-deprived nervous systems in rats may affect their response at the sensorimotor pathways and respective controlling centers with sex differences, which were related to the used stimulus. (AU)

• Processo FAPESP: 15/18415-8 - Diferenças de gênero no desenvolvimento ontogenético, comportamento e metabolismo energético em ratos submetidos à anóxia neonatal: enfoque no hipocampo, hipotálamo,leptina e células da glia .
• Beneficiário: Maria Inês Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22313-3 - Influência da ausência da melatonina maternal durante a gestação e lactação sobre a neurogênese e sobre o desenvolvimento somático e sensório-motor da prole.
• Beneficiário: Lívia Clemente Motta Teixeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/05218-5 - Efeito da fotobioestimulação em diferentes neuropatias periférica em ratos.
• Beneficiário: Marucia Chacur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/24256-0 - Estudo das estruturas envolvidas na dor orofacial e perspectivas do tratamento combinado de laser de baixa intensidade e vitaminas do complexo B em modelo de neuralgia trigeminal
• Beneficiário: Daniel de Oliveira Martins
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado