Multidimensional Approach Assessing the Role of Interleukin 1 Beta in Mesial Temporal Lobe Epilepsy

Autor(es):Mostrar menos -

Santos, Renato O. ^{[1, 2]} ; Secolin, Rodrigo ^{[1, 2]} ; Barbalho, Patricia G. ^{[1, 2]} ; Silva-Alves, Mariana S. ^{[1, 2]} ; Alvim, Marina K. M. ^{[3, 1]} ; Yasuda, Clarissa L. ^{[3, 1]} ; Rogerio, Fabio ^{[4, 1]} ; Velasco, Tonicarlo R. ^[5] ; Sakamoto, Americo C. ^[5] ; Teixeira, Antonio L. ^[6] ; Cendes, Fernando ^{[3, 1]} ; Maurer-Morelli, V, Claudia ; Lopes-Cendes, Iscia ^{[7, 8]}

Afiliação do(s) autor(es)

^[1] Brazilian Inst Neurosci & Neurotechnol, Campinas - Brazil

^[2] Univ Estadual Campinas, Dept Translat Med, Campinas - Brazil

^[3] Univ Estadual Campinas, Dept Neurol, Campinas - Brazil

^[4] Univ Estadual Campinas, Dept Pathol, Campinas - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav Sci, Ribeirao Preto - Brazil

^[6] Univ Fed Minas Gerais, Dept Internal Med, Belo Horizonte, MG - Brazil

^[7] Maurer-Morelli, Claudia, V, Univ Estadual Campinas, Dept Translat Med, Campinas - Brazil

^[8] Maurer-Morelli, Claudia, V, Brazilian Inst Neurosci & Neurotechnol, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN NEUROLOGY; v. 12, AUG 5 2021.
• Citações Web of Science: 0

Resumo

We aimed to investigate the role of interleukin-1 beta (IL-1 beta) in the mechanisms underlying mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE+HS). We assessed a cohort of 194 patients with MTLE+HS and 199 healthy controls. Patients were divided into those with positive and negative antecedent febrile seizures (FS). We used a multidimensional approach, including (i) genetic association with single nucleotide polymorphisms (SNPs) in the IL1B gene; (ii) quantification of the IL1B transcript in the hippocampal tissue of patients with refractory seizures; and (iii) quantification of the IL-1 beta protein in the plasma. We found a genetic association signal for two SNPs, rs2708928 and rs3730364{*}C in the IL1B gene, regardless of the presence of FS (adjusted p = 9.62e-11 and 5.14e-07, respectively). We found no difference between IL1B transcript levels when comparing sclerotic hippocampal tissue from patients with MTLE+HS, without FS, and hippocampi from autopsy controls (p > 0.05). Nevertheless, we found increased IL-1 beta in the plasma of patients with MTLE+HS with FS compared with controls (p = 0.0195). Our results support the hypothesis of a genetic association between MTLE+HS and the IL1B gene (AU)

• Processo FAPESP: 08/52590-8 - Avaliação da importância dos genes ptprm e IL1-b na epilepsia de lobo temporal mesial com atrofia hipocampal através da genotipagem de SNPs
• Beneficiário: Renato Oliveira dos Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/08526-8 - Avaliação da arquitetura genômica ancestral em pacientes com epilepsia focal e generalizada
• Beneficiário: Rodrigo Secolin
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 13/07559-3 - Instituto Brasileiro de Neurociência e Neurotecnologia - BRAINN
• Beneficiário: Fernando Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs