Synergistic effect of photobiomodulation and phthalocyanine photosensitizer on fibroblast signaling responses in an in vitro three-dimensional microenvironment

Autor(es)

Souza, Carla ^[1] ; Jayme, Cristiano Ceron ^[1] ; Rezende, Nayara ^[1] ; Tedesco, Antonio Claudio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Chem, Ctr Nanotechnol & Tissue Engn, Photobiol & Photomed Res Grp, Fac Philosophy Sci &, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 222, SEP 2021.
• Citações Web of Science: 0

Resumo

Photobiomodulation (PBM) is a promising medical treatment modality in the area of photodynamic therapy (PDT). In this study, we investigated the effect of combined therapy in a 3D microenvironment using aluminum chloride phthalocyanines (AlClPc) as the photosensitizing agent. Normal human fibroblast-containing collagen biomatrix was prepared and treated with an oil-in-water (o/a) AlClPc-loaded nanoemulsion (from 0.5 to 3.0 mu M) and irradiated at a range of fluences (from 0.1 to 3.0 J/cm2) using a continuous-wave light-emitting diode (LED) irradiation system (660 nm). PBM at 1.2 J/cm2 and AlClPc/NE at 0.5 mu M modified the fibroblast signaling response under 3D conditions, promoting collagen synthesis, ROS production, MMP-9 secretion, proliferation of the actin network, and facile myofibroblastic differentiation. PBM alone (at 1.2 J/cm2 and 0.3 J/cm2) had no significant effect on any of these parameters. The combined therapy affected myofibroblastic differentiation, inflammatory response, and extracellular matrix pliability, and should thus be examined further in subsequent studies considering that no side effects of PBM have been reported. Even though significant progress has been made in the field of phototherapy in recent years, it is necessary to further elucidate the detailed mechanisms underlying its effects already shown in 2D conditions to increase the acceptance of this beneficial and noninvasive therapeutic approach. (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/10237-1 - Desenvolvimento e caracterização de scaffolds à base de PCL-PGE e DNA aplicados à engenharia de tecidos para regeneração óssea
• Beneficiário: Cristiano Ceron Jayme
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/01272-5 - Modelos in vitro de pele reconstituída: desenvolvimento, avaliação dos danos e da eficácia fotoprotetora nas regiões UVA e visível do espectro solar
• Beneficiário: Carla Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado