Frequency and characterization of RHD variant alleles in a population of blood donors from southeastern Brazil: Comparison with other populations

Autor(es)

Rodrigues, Evandra Strazza ^{[1, 2]} ; Romagnoli, Aline Cristina ^{[1, 2]} ; Santos, Flavia Leite Souza ^{[1, 2]} ; Cutter, Talitha Baldin ^{[1, 2]} ; Catelli, Lucas Ferioli ^{[1, 2]} ; Cedric, Vrignaud ^[3] ; Peyrard, Thierry ; Covas, Dimas Tadeu ^{[1, 2, 4]} ; de Castilho, Lilian Maria ^[5] ; Kashima, Simone ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Res, Ave Tenente Catao Roxo, BR-2501 Ribeirao Preto, SP - Brazil

^[3] Inst Natl Transfus Sanguine INTS, Dept Ctr Natl Refere Grp Sanguins, Paris - France

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Clin Med, Ribeirao Preto, SP - Brazil

^[5] Lab Imunohematol Mol Hemocent Unicamp, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TRANSFUSION AND APHERESIS SCIENCE; v. 60, n. 4 AUG 2021.
• Citações Web of Science: 0

Resumo

Background: The correct determination of D antigen could help to avoid alloimmunization in pregnant women and patients receiving blood transfusions. However, there are limitations in the identification of D variants as the partial and weak D phenotypes make the determination of D antigen a great challenge in the transfusion routine.' Study design and methods: The molecular characterization of D variants was performed on blood donors from southeastern Brazil with atypical D typing. Furthermore, the serological profile of all RHD variant alleles identified was analyzed using different Anti-D clones. The prevalence of RHD alleles and genotypes found was compared with those described in other countries and in other regions from Brazil. Results: Atypical serologic D typing occurred in 0.79 % of blood donors. The majority of RHD variant alleles (88 %) were first characterized by multiplex PCR and PCR-SSP as RHD{*}weak partial 4 (47 %), followed by RHD{*}weak D type 3 (29.9 %), RHD{*}weak D type 2 (3.9 %) and RHD{*}weak D type 1 (3.1 %). Genomic DNA sequencing characterized the RHD{*}weak partial 4 variants found in RHD{*}DAR1.2 (weak 4.2.2) (22 %), RHD{*}DAR3 (weak 4.0.1) (2.4 %), RHD{*}DAR3.1 (weak 4.0) (22 %) and RHD{*}DAR4 (weak 4.1) (0.8 %). RHD variant alleles associated with partial D, such as, RHD{*}DAU-4 (1.6 %), RHD{*}DAU-5 (2.4 %), RHD{*}DAU-6 (1.6 %), RHD{*} DIII type 8 (1.6 %), RHD{*}DVII (3.9 %) and RHD{*} DMH (0.8 %) were also observed. Conclusion: The prevalence of RHD variant alleles observed in this cohort differ from those found in other populations, including Brazilians from other regions. RHD allele distribution in specific regions should be considered for implementation of algorithms and genotyping strategies aiming at a more effective and safe transfusion. (AU)

• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/26950-6 - Aloimunização eritrocitária na doença falciforme: identificação de variantes do sistema rh e de marcadores imunológicos
• Beneficiário: Evandra Strazza Rodrigues Sandoval
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado