Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

Autor(es)

Pinheiro, Vanessa Elisa ^{[1, 2]} ; Horvath, Ilona Sarvari ^[2] ; Lundin, Magnus ^[2] ; Polizeli, Maria de Lourdes Teixeira de Moraes ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Ribeirao Preto - Brazil

^[2] Univ Boras, Swedish Ctr Resource Recovery, Boras - Sweden

^[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ave 3-900, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 15, n. 5, p. 1447-1460, SEP 2021.
• Citações Web of Science: 3

Resumo

Biofuels contribute environment-friendly, renewable energy, minimizing dependence on fossil energy. The pretreatment of biomass is a practical step that accelerates and facilitates the hydrolysis of complex materials. This work aimed to screen, select, and study cocktail formulations for biomass hydrolysis, where the enzymes were provided both from a commercial source (Trichoderma reesei, Novozymes (R)) and through the cultivation of Aspergillus brasiliensis and Aspergillus tamarii Kita. Experimental mixture designs were used to optimize the enzymatic conversion of substrates into simple sugars. A crude extract rich in amylase (AAB) had a significant favorable influence on cornmeal hydrolysis by maximizing the yield of reducing sugars (RS) (173 mu mol mL(-1)). Celluclast (TM), rich in cellulase, significantly affected the hydrolysis of banana peel, maximizing the RS yield (175 mu mol mL(-1)). Variable degrees of enzyme synergism were evident from statistical analysis of the biomass hydrolysis. (c) 2021 Society of Chemical Industry and John Wiley \& Sons, Ltd (AU)

• Processo FAPESP: 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 18/07522-6 - Desenvolvimento de tecnologias ecológicas para a hidrólise de biomassa e resíduos da indústria de celulose e papel
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático