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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

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Author(s):
Pinheiro, Vanessa Elisa [1, 2] ; Horvath, Ilona Sarvari [2] ; Lundin, Magnus [2] ; Polizeli, Maria de Lourdes Teixeira de Moraes [1, 3]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Ribeirao Preto - Brazil
[2] Univ Boras, Swedish Ctr Resource Recovery, Boras - Sweden
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ave 3-900, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 15, n. 5, p. 1447-1460, SEP 2021.
Web of Science Citations: 3
Abstract

Biofuels contribute environment-friendly, renewable energy, minimizing dependence on fossil energy. The pretreatment of biomass is a practical step that accelerates and facilitates the hydrolysis of complex materials. This work aimed to screen, select, and study cocktail formulations for biomass hydrolysis, where the enzymes were provided both from a commercial source (Trichoderma reesei, Novozymes (R)) and through the cultivation of Aspergillus brasiliensis and Aspergillus tamarii Kita. Experimental mixture designs were used to optimize the enzymatic conversion of substrates into simple sugars. A crude extract rich in amylase (AAB) had a significant favorable influence on cornmeal hydrolysis by maximizing the yield of reducing sugars (RS) (173 mu mol mL(-1)). Celluclast (TM), rich in cellulase, significantly affected the hydrolysis of banana peel, maximizing the RS yield (175 mu mol mL(-1)). Variable degrees of enzyme synergism were evident from statistical analysis of the biomass hydrolysis. (c) 2021 Society of Chemical Industry and John Wiley \& Sons, Ltd (AU)

FAPESP's process: 18/07522-6 - Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry
Grantee:Maria de Lourdes Teixeira de Moraes Polizeli
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
Grantee:Marcos Silveira Buckeridge
Support Opportunities: Research Projects - Thematic Grants