Milk lymphocyte profile and macrophage functions: new insights into the immunity of the mammary gland in quarters infected with Corynebacterium bovis

Autor(es):Mostrar menos -

Silva, Vitoria M. ^[1] ; Souza, Marina T. ^{[1, 2]} ; Blagitz, Maiara G. ^{[3, 2]} ; Souza, Fernando N. ^{[1, 2]} ; Batista, Camila F. ; Alves, Alexandre J. ^[1] ; Fernandes, Artur C. C. ^{[3, 1]} ; Sanchez, Eduardo M. R. ^{[4, 5]} ; Ordinola-Ramirez, Carla M. ^[5] ; da Costa, Luciana ^[6] ; Della Libera, Alice M. M. P. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Paraiba, Ctr Ciencias Agr, Dept Ciencias Vet, Nucleo Aplicado Prod & Sanidade Glandula Mamaria, BR-58397000 Areia - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Vet Clin Immunol Res Grp, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

^[3] Univ Fed Paraiba, Programa Posgrad Ciencia Anim, BR-58397000 Areia - Brazil

^[4] Univ Sao Paulo, Inst Med Trop, Lab Sorol & Imunobiol, BR-05403000 Sao Paulo - Brazil

^[5] Univ Nacl Toribio Rodriguez Mendoza Amazonas, Fac Ciencias Salud, Dept Salud Publ, Chachapoyas 01000 - Peru

^[6] Ohio State Univ, Dept Vet Prevent Med, Coll Vet Med, Columbus, OH 43210 - USA

• Tipo de documento: Artigo Científico
• Fonte: BMC Veterinary Research; v. 17, n. 1 AUG 25 2021.
• Citações Web of Science: 0

Resumo

Backgrounds The present study explored the viability of bovine milk macrophages, their intracellular production of reactive oxygen and nitrogen species (RONS), and their phagocytosis of Staphylococcus aureus, as well as the profile of lymphocytes, from healthy udder quarters and udder quarters infected by Corynebacterium bovis. The study included 28 healthy udder quarters from 12 dairy cows and 20 udder quarters infected by C. bovis from 10 dairy cows. The percentages of macrophages and lymphocytes were identified by flow cytometry using monoclonal antibodies. Macrophage viability, RONS production, and S. aureus phagocytosis were evaluated by flow cytometry. Results Milk samples from quarters infected with C. bovis showed a lower percentage of macrophages but an increased number of milk macrophages per mL and a higher percentage of macrophages that produced intracellular RONS and phagocytosed S. aureus. No effect of C. bovis infection on macrophage viability was found. Udder quarters infected by C. bovis showed a higher percentage of T cells and CD4(+) T lymphocytes, but no effect was found on the percentage of CD8(+) CD4(-) T, CD8(-) CD4(-) T, or B lymphocytes. Conclusions Thus, our results corroborate, at least in part, the finding that intramammary infections by C. bovis may offer protection against intramammary infections by major pathogens. (AU)

• Processo FAPESP: 09/50672-0 - Avaliação funcional dos leucócitos sanguíneos e lácteos de vacas naturalmente infectados pelo vírus da leucose dos bovinos
• Beneficiário: Alice Maria Melville Paiva Della Libera
• Modalidade de apoio: Auxílio à Pesquisa - Regular