Breast Cancer Targeting of a Drug Delivery System through Postsynthetic Modification of Curcumin@N-3-bio-MOF-100 via Click Chemistry

Autor(es)

Alves, Renata C. ^[1] ; Schulte, Zachary M. ^[2] ; Luiz, Marcela T. ^[3] ; da Silva, Patricia Bento ^[4] ; Frem, Regina C. G. ^[5] ; Rosi, Nathaniel L. ^[2] ; Chorilli, Marlus ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Drugs & Med, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

^[2] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 - USA

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Brasilia UnB, Inst Biol Sci, Dept Genet & Morphol, BR-70910900 Brasilia, DF - Brazil

^[5] Sao Paulo State Univ UNESP, Inst Chem, BR-14800970 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Inorganic Chemistry; v. 60, n. 16, p. 11739-11744, AUG 16 2021.
• Citações Web of Science: 1

Resumo

Metal-organic frameworks (MOFs) offer many opportunities for applications across biology and medicine. Their wide range of chemical composition makes toxicologically acceptable formulation possible, and their high level of functionality enables possible applications as delivery systems for therapeutics agents. Surface modifications have been used in drug delivery systems to minimize their interaction with the bulk, improving their specificity as targeted carriers. Herein, we discuss a strategy to achieve a tumor-targeting drug-loaded MOF using ``click{''} chemistry to anchor functional folic acid (FA) molecules on the surface of N-3-bio-MOF-100. Using curcumin (CCM) as an anticancer drug, we observed drug loading encapsulation efficiencies (DLEs) of 24.02 and 25.64% after soaking N-3-bio-MOF-100 in CCM solutions for 1 day and 3 days, respectively. The success of postsynthetic modification of FA was confirmed by H-1 NMR spectroscopy, Fourier transform infrared spectroscopy (FTIR), and liquid chromatography-mass spectrometry (LC-MS). The stimuli-responsive drug release studies demonstrated an increase of CCM released under acidic microenvironments. Moreover, the cell viability assay was performed on the 4T1 (breast cancer) cell line in the presence of CCM@N-3-bio-MOF-100 and CCM@N-3-bio-MOF-100/FA carriers to confirm its biological compatibility. In addition, a cellular uptake study was conducted to evaluate the targeting of tumor cells. (AU)

• Processo FAPESP: 17/26065-2 - Encapsulação de curcumina em BioMOF-100 funcionalizado com folato: desenvolvimento e avaliação do potencial no tratamento do Câncer de Mama
• Beneficiário: Renata Carolina Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/26405-0 - Modificação de superfície com ácido fólico em metal-organic frameworks mesoporos carregados com curcumina através de reações click
• Beneficiário: Renata Carolina Alves
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado