Endocrine disruption caused by the aquatic exposure to aluminum and manganese in Astyanax altiparanae (Teleostei: Characidae) females during the final ovarian maturation

Autor(es)

Correia, Tiago Gabriel ^[1] ; Vieira, Vanessa Aparecida Rocha Oliveira ^[1] ; Narcizo, Amanda de Moraes ^[1] ; Zampieri, Ricardo Andrade ^[1] ; Floeter-Winter, Lucile Maria ^[1] ; Moreira, Renata Guimaraes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 249, NOV 2021.
• Citações Web of Science: 1

Resumo

Aluminum (Al) and manganese (Mn) can be toxic to aquatic biota and cause endocrine disruption in fish, affecting reproduction. This study evaluates the physiological responses of the ray-finned teleost fish Astyanax altiparanae vitellogenic females after acute exposure (96 h) to Al and Mn (alone and combined) in acid pH followed by the same period of exposure to metal-free water in neutral pH. The aim of this second period of exposure was to assess the recovery capacity from the toxic effects these metals. Five experimental groups were established: a control in neutral pH (Ctrl), and acidic pH (Ac), aluminum (Al), manganese (Mn), and Al + Mn groups, maintaining the acidic pH in the groups to which metals were added. The following biological parameters were evaluated: metal tissue concentration, relative fecundity (RF: absolute fecundity/body mass). Plasma levels of cortisol (proxy for stress) and 17 alpha hydroxyprogesterone (17 alpha-OHP), and gene expression of pituitary lh beta mRNA (proxies for final maturation) were measured to evaluate endocrine disruption. In the synchronic exposure, the presence of Mn potentiated the accumulation of Al in gills. The females from acidic pH and Al groups showed a reduced RF. Exposure to Al and Mn triggered an endocrine disruption response, evidenced by a decrease in the plasma concentration of 17 alpha-OHP and cortisol. Despite this anti-steroidogenic effect, no changes occurred in the pituitary gene expression of lh beta. The endocrine changes and the metal accumulation were temporary, while the impacts on RF under the experimental conditions suggest permanent impairment in the reproduction of this species. (AU)

• Processo FAPESP: 08/57687-0 - Effects of global climate change of the Brazilian fauna: a conservation physiology approach
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático