Development and Validation of a Method for Quantification of 28 Psychotropic Drugs in Postmortem Blood Samples by Modified Micro-QuEChERS and LC-MS-MS

Autor(es)

Rodrigues, Tais B. ^[1] ; Morais, Damila R. ^[2] ; Gianvecchio, Victor A. P. ^[3] ; Aquino, Elvis M. ^[3] ; Cunha, Ricardo L. ^[4] ; Huestis, Marilyn A. ^[5] ; Costa, Jose Luiz ^{[6, 1]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Campinas Poison Control Ctr, BR-13083859 Campinas, SP - Brazil

^[2] Shimadzu Brazil, Analyt Div, BR-06460000 Barueri, SP - Brazil

^[3] Sao Paulo State Police, Forens Toxicol Lab, Inst Legal Med, BR-05507060 Sao Paulo, SP - Brazil

^[4] Inst Anal & Forens Res, Forens Toxicol Lab, BR-49100000 Sao Cristovao, Sergipe - Brazil

^[5] Thomas Jefferson Univ, Lambert Ctr Study Med Cannabis & Hemp, Inst Emerging Hlth Profess, Philadelphia, PA 19107 - USA

^[6] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ANALYTICAL TOXICOLOGY; v. 45, n. 7, p. 644-656, SEP 2021.
• Citações Web of Science: 0

Resumo

The development of new sample preparation alternatives in analytical toxicology leading to quick, effective, automated and environmentally friendly procedures is growing in importance. One of these alternatives is the QuEChERS, originally developed for the analysis of pesticide residues, producing cleaner extracts than liquid-liquid extraction, and easier separation of aqueous and organic phases. However, there are few published studies on the miniaturization of this technique for forensic toxicology, especially in postmortem analysis. We developed and validated a modified micro-QuEChERS and LC-MS-MS assay to quantify 16 antidepressants, 7 antipsychotics and 3 metabolites and semi-quantify norfluoxetine and norsertraline in postmortem blood. The calibration curve was linear from 1 to 500 ng/mL, achieved an r>0.99, with all standards quantifying within +/- 15% of target except +/- 20% at the limit of quantification of 1 ng/mL for 26 substances. The F test was applied to evaluate if the variance between replicates remained constant for all calibrators. Six weighting factors were analyzed (1/x, 1/x(2), 1/x(0,5), 1/y, 1/y(2) and 1/y(0,5)), with the weighting factor with the lowest sum of residual regression errors (1/x(2)) selected. No endogenous or exogenous interferences were observed. Method imprecision and bias were <19.0% and 19.7%, respectively. Advantages of this method include a low sample volume of 100 mu L, simple but effective sample preparation and a rapid 8.5-min run time. The validated analytical method was successfully applied to the analysis of 100 authentic postmortem samples. (AU)

• Processo FAPESP: 18/00432-1 - A Toxicologia das Novas Substâncias Psicoativas (NSP): epidemiologia do consumo através da análise de amostras de cabelo e fluido oral
• Beneficiário: José Luiz da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular