Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Hormetic effects of low-dose gamma rays in soybean seeds and seedlings: A detection technique using optical sensors

Texto completo
Autor(es):
Oliveira, Nielsen Moreira [1] ; de Medeiros, Andre Dantas [2] ; Nogueira, Marina de Lima [3] ; Arthur, Valter [4] ; Mastrangelo, Thiago de Araujo [4] ; da Silva, Clissia Barboza [4]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Dept Crop Sci, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Fed Vicosa, Dept Agron, BR-36570900 Vicosa, MG - Brazil
[3] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP - Brazil
[4] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13416000 Piracicaba, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: COMPUTERS AND ELECTRONICS IN AGRICULTURE; v. 187, AUG 2021.
Citações Web of Science: 0
Resumo

The use of radiation technology in agriculture has increased remarkably in recent years. The stimulating effect of low-dose gamma rays, known as hormesis, improves the adaptive response of living organisms to more severe challenges. Different areas of agriculture such as plant physiology, crop breeding and seed biology demand accurate methods for real-time diagnosis of the ionizing radiation effect on biological systems. However, traditional measures are still calculated from slow, laborious and destructive techniques, which depend on human vision. Here, we present two robust optical sensors based on multispectral and fluorescence imaging approaches for rapid and non-destructive detection of the hormetic effects of low-dose gamma radiation in soybean seeds and seedings. Multispectral images were acquired from seeds treated with gamma radiation (0, 12, 16 and 20 Gy), and the reflectance, autofluorescence, texture and color features were extracted from images. Next, a chlorophyll fluorescence-based technique was applied to measure the minimal level of fluorescence (F-0), maximum fluorescence (F-M), average chlorophyll a fluorescence and quantum yield of photosystem II (F-V/F-M) in seedlings grown from irradiated and non-irradiated seeds. Using a reflectance technology, the seedlings were also evaluated for anthocyanin, chlorophyll a and normalized difference vegetation index (NDVI). Results showed that multispectral and fluorescence-based techniques provide useful information on the hormetic effects of gamma rays in soybean seeds and seedlings, which are invisible to the naked eye. Our findings provide a better understanding of the link between spectral and fluorescence parameters and the hormetic effect of low-dose gamma rays. From a practical point of view, such optical sensors can provide markers for fast, non-destructive and reliable identification of the best gamma-ray doses to stimulate soybean growth parameters. However, we strongly support in-depth studies of the dose-response relationship in seeds with different initial physiological potential from different soybean genotypes. (AU)

Processo FAPESP: 18/03802-4 - EMU concedido no processo 2017/15220-7: sistema de imagem VideoMeterLab
Beneficiário:Clíssia Barboza da Silva
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 18/03793-5 - EMU concedido no processo 2017/15220-7: sistema de imagem SeedReporter câmera spectral & colour
Beneficiário:Clíssia Barboza da Silva
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 17/15220-7 - Métodos de análise de imagens não destrutivos para avaliação da qualidade de sementes
Beneficiário:Clíssia Barboza da Silva
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 18/01774-3 - Métodos de análise de imagens não destrutivos para avaliação da qualidade de sementes
Beneficiário:Clíssia Barboza da Silva
Linha de fomento: Bolsas no Brasil - Apoio a Jovens Pesquisadores