Shedding light on toxicity of SARS-CoV-2 peptides in aquatic biota: A study involving neotropical mosquito larvae (Diptera: Culicidae)

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Mendonca-Gomes, Juliana Moreira ^[1] ; Charlie-Silva, Ives ^[2] ; Guimaraes, Abraao Tiago Batista ^[3] ; Estrela, Fernanda Neves ^[3] ; Calmon, Marilia Freitas ^[4] ; Miceli, Rafael Nava ^[5] ; Sanches, Paulo R. S. ^[6] ; Bittar, Cintia ^[4] ; Rahal, Paula ^[4] ; Cilli, Eduardo M. ^[6] ; Ahmed, Mohamed Ahmed Ibrahim ^[7] ; Vogel, Christoph F. A. ^{[8, 9]} ; Malafaia, Guilherme ^{[3, 10, 11]}

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^[1] Univ Sao Paulo, Dept Imunol, Inst Ciencias Biomed, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Farmacol, Inst Ciencias Biomed, Sao Paulo, SP - Brazil

^[3] Inst Fed Goiano, Programa Pos Grad Conservacao Recursos Nat Cerr, Urutai, Go - Brazil

^[4] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP - Brazil

^[5] SeMAE Serv Municipal Autonomo Agua & Esgoto, Sao Paulo, SP - Brazil

^[6] Univ Estadual Paulista, Inst Quim, Araraquara, SP - Brazil

^[7] Assiut Univ, Dept Plant Protect, Fac Agr, Assiut 71526 - Egypt

^[8] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 - USA

^[9] Univ Calif Davis, Ctr Hlth & Environm, Davis, CA 95616 - USA

^[10] Univ Fed Goias, Programa Pos Grad Biotecnol & Biodiversida, Goiania, Go - Brazil

^[11] Univ Fed Uberlancia, Programa Pos Grad Ecol & Conservacao Recursos Nat, Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Pollution; v. 289, NOV 15 2021.
• Citações Web of Science: 0

Resumo

Knowledge about how the COVID-19 pandemic can affect aquatic wildlife is still extremely limited, and no effect of SARS-CoV-2 or its structural constituents on invertebrate models has been reported so far. Thus, we investi-gated the presence of the 2019-new coronavirus in different urban wastewater samples and, later, evaluated the behavioral and biochemical effects of the exposure of Culex quinquefasciatus larvae to two SARS-CoV-2 spike protein peptides (PSPD-2002 and PSPD-2003) synthesized in our laboratory. Initially, our results show the contamination of wastewater by the new coronavirus, via RT-qPCR on the viral N1 gene. On the other hand, our study shows that short-term exposure (48 h) to a low concentration (40 mu g/L) of the synthesized peptides induced changes in the locomotor and the olfactory-driven behavior of the C. quinquefascitus larvae, which were asso-ciated with increased production of ROS and AChE activity (cholinesterase effect). To our knowledge, this is the first study that reports the indirect effects of the COVID-19 pandemic on the larval phase of a freshwater invertebrate species. The results raise concerns at the ecological level where the observed biological effects may lead to drastic consequences. (AU)

• Processo FAPESP: 19/19939-1 - Efeitos de fármacos marinhos em células ZFL
• Beneficiário: Ives Charlie da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 20/05761-3 - Estudo da ação de peptídeos sintéticos como antivirais contra o SARS-CoV-2 (COVID-19) e avaliação combinada com anti-inflamatórios comerciais
• Beneficiário: Eduardo Maffud Cilli
• Modalidade de apoio: Auxílio à Pesquisa - Regular