Interrelationship between the Microbial Communities of the Root Canals and Periodontal Pockets in Combined Endodontic-Periodontal Diseases

Autor(es)

Lopes, Erica M. ^[1] ; Passini, Maicon R. Z. ^[1] ; Kishi, Luciano T. ^[2] ; Chen, Tsute ^[3] ; Paster, Bruce J. ^[4] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Div Endodont, BR-13400001 Piracicaba, SP - Brazil

^[2] Natl Lab Sci Comp, BR-25715183 Petropolis, RJ - Brazil

^[3] Forsyth Inst, Dept Mol Genet, Cambridge, MA 02142 - USA

^[4] Forsyth Inst, Microbiol Dept, Cambridge, MA 02142 - USA

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 9, n. 9 SEP 2021.
• Citações Web of Science: 0

Resumo

Periodontal and Endodontic diseases are biofilm-related diseases. The presence of microorganisms in root canals (RCs) and the complex microbiota of periodontal pockets (PPs) contribute to the development of endodontic-periodontal diseases. This study performed a systemic analysis using state-of-the-art sequence data to assess the microbial composition of infected RCs and PPs to further assess the microbiota and verify the possibility of cross-infection between these sites. The microbiomes of these combined diseases were examined with a focus on the V3-V4 hypervariable region of the 16S rRNA gene. The number of species in PP was higher than in RC, and there was a predominance of obligate anaerobes and gram-negative bacteria. In the RCs, the genera Enterococcus, Parvimonas, Stomatobaculum predominated, in contrast, the PPs revealed a predominance of Enterococcus, Parvimonas, Stomatobaculum, Peptostreptococcus and Mogibacterium. The RC and PP microbiome was not similar with regards to the sharing of OTUs for phyla and genera (8 and 67, respectively). The evaluation of molecular markers revealed a large number of markers for resistance to antibiotics of the carbapenem and beta-lactam type (broad spectrum). Another relevant finding of this study was the markers related to systemic diseases related to cardiac muscle and rheumatology, among others. In conclusion, the RC microbiota was less complex and diverse than PP. Interactions between microbial communities were present. The shared genus can signal communication between the endodontic and periodontal microbiomes. (AU)

• Processo FAPESP: 15/23479-5 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/14448-0 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
• Beneficiário: Erica Mendes Lopes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado