The Labile Iron Pool Reacts Rapidly and Catalytically with Peroxynitrite

Autor(es)

Condeles, Andre Luis ^[1] ; Toledo Junior, Jose Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMOLECULES; v. 11, n. 9 SEP 2021.
• Citações Web of Science: 0

Resumo

While investigating peroxynitrite-dependent oxidation in murine RAW 264.7 macrophage cells, we observed that removal of the Labile Iron Pool (LIP) by chelation increases the intracellular oxidation of the fluorescent indicator H2DCF, so we concluded that the LIP reacts with peroxynitrite and decreases the yield of peroxynitrite-derived oxidants. This was a paradigm-shifting finding in LIP biochemistry and raised many questions. In this follow-up study, we address fundamental properties of the interaction between the LIP and peroxynitrite by using the same cellular model and fluorescence methodology. We have identified that the reaction between the LIP and peroxynitrite has catalytic characteristics, and we have estimated that the rate constant of the reaction is in the range of 10(6) to 10(7) M(-1)s(-1). Together, these observations suggest that the LIP represents a constitutive peroxynitrite reductase system in RAW 264.7 cells. (AU)

• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs