olecular basis of a bacterial-amphibian symbiosis revealed by comparative genomics, modeling, and functional testin

Autor(es)

Brunetti, Andres E. ^{[1, 2]} ; Bunk, Boyke ^[3] ; Lyra, Mariana L. ^{[4, 5]} ; Fuzo, Carlos A. ^[6] ; Marani, Mariela M. ^[7] ; Sproeer, Cathrin ^[3] ; Haddad, Celio F. B. ^{[4, 5]} ; Lopes, Norberto P. ^[1] ; Overmann, Joerg ^{[3, 8]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias BioMol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Nacl Misiones, Fac Ciencias Exactas, Inst Biol Subtrop CONICET UNaM, Lab Genet Evolut, RA-3300 Posadas - Argentina

^[3] Leibniz Inst DSMZ German Collect Microorganisms &, D-38124 Braunschweig - Germany

^[4] Univ Estadual Paulista, Ctr Aquicultura, Inst Biociencias, BR-13506900 Rio Claro, SP - Brazil

^[5] Univ Estadual Paulista, Dept Biodiversidade, Inst Biociencias, BR-13506900 Rio Claro, SP - Brazil

^[6] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[7] Consejo Nacl Invest Cient & Tecn, Inst Patagon Estudio Ecosistemas Continentales, IPEEC CONICET, U9120ACD, Puerto Madryn - Argentina

^[8] Tech Univ Carolo Wilhelmina Braunschweig, Mikrobiol, D-38106 Braunschweig - Germany

• Tipo de documento: Artigo Científico
• Fonte: ISME Journal; v. 16, n. 3 OCT 2021.
• Citações Web of Science: 1

Resumo

The molecular bases for the symbiosis of the amphibian skin microbiome with its host are poorly understood. Here, we used the odor-producer Pseudomonas sp. MPFS and the treefrog Boana prasina as a model to explore bacterial genome determinants and the resulting mechanisms facilitating symbiosis. Pseudomonas sp. MPFS and its closest relatives, within a new clade of the P. fluoresens Group, have large genomes and were isolated from fishes and plants, suggesting environmental plasticity. We annotated 16 biosynthetic gene clusters from the complete genome sequence of this strain, including those encoding the synthesis of compounds with known antifungal activity and of odorous methoxypyrazines that likely mediate sexual interactions in Boana prasina. Comparative genomics of Pseudomonas also revealed that Pseudomonas sp. MPFS and its closest relatives have acquired specific resistance mechanisms against host antimicrobial peptides (AMPs), specifically two extra copies of a multidrug efflux pump and the same two-component regulatory systems known to trigger adaptive resistance to AMPs in P. aeruginosa. Subsequent molecular modeling indicated that these regulatory systems interact with an AMP identified in Boana prasina through the highly acidic surfaces of the proteins comprising their sensory domains. In agreement with a symbiotic relationship and a highly selective antibacterial function, this AMP did not inhibit the growth of Pseudomonas sp. MPFS but inhibited the growth of another Pseudomonas species and Escherichia coli in laboratory tests. This study provides deeper insights into the molecular interaction of the bacteria-amphibian symbiosis and highlights the role of specific adaptive resistance toward AMPs of the hosts. (AU)

• Processo FAPESP: 20/02207-5 - Inventariando o metabolismo secundário através da metabolômica: contribuição para a valoração da biodiversidade brasileira
• Beneficiário: Norberto Peporine Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
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• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 17/23725-1 - Análise genômico de bactérias simbióticas de pele em pererecas como fonte potencial de compostos voláteis
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• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado