The effect of association of aspirin and omega 3 in rat temporomandibular joint with induced arthritis

Autor(es)

Ceotto, Beatriz H. ^[1] ; Figueroba, Sidney R. ^[1] ; Ferreira, Luiz Eduardo N. ^[2] ; Amorim, Klinger S. ^[1] ; Sanchez, Jonny B. ^[1] ; Gercina, Anne Caroline ^[1] ; dos Santos, Victor Augusto B. ^[1] ; Groppo, Francisco C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, UNICAMP Piracicaba, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Guarulhos, Lab Inflammat & Immunol, Guarulhos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER; v. 239, JAN 2022.
• Citações Web of Science: 0

Resumo

This study aimed to evaluate the effects of omega-3 (w3) polyunsaturated fatty acids, in association with aspirin (AA), on the morphology of cytokine release in the temporomandibular joint (TMJ) of rats induced with rheumatoid arthritis (IR) by injecting 100 mu L of complete Freund's adjuvant with bovine type II collagen at the tail base. Thirty-two adult male rats were divided into treatment groups: Sham, treated with 0.9% sodium chloride (NaCl) p.o.; IR-control, treated with 0.9% NaCl p.o.; IR-w3 treated with w3 PUFAS (85 mg/kg/day p.o.); and IR-w3 + AA treated with w3 (85 mg/kg/day p.o.) + AA (20 mg/kg/day i.p.). After maintained treatment for seven days, the animals were euthanized. Bilateral TMJs from each rat were removed and one was subjected to histological immunoassays and enzyme-linked immunosorbent assays to assess interleukin (IL)-113, tumor necrosis factor-a, and IL-10 levels. Data analysis was performed using the Kruskal-Wallis and Dunn tests. In the IR-w3 and IR-w3 + AA groups, the TMJ was greater than in the IR-control group (P < 0.0001). The addition of AA did not improve the effects of w3 (P = 0.0698). Similarly, the addition of AA conferred no additional effects on the cytokine levels (P > 0.05); however, it increased the proteoglycan density, compared with w3 alone. We found that w3 exhibited anti-inflammatory activity in arthritic rats, and the addition of AA increased proteoglycan density, but did not affect cytokine expression. (c) 2021 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 17/17472-3 - Efeito da associação do ácido acetilsalicílico com ômega 3 no processo inflamatório induzido por CFA na articulação temporomandibular (ATM) de ratos: estudo piloto
• Beneficiário: Beatriz Hidalgo Ceotto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica