| Full text | |
| Author(s): |
Ceotto, Beatriz H.
[1]
;
Figueroba, Sidney R.
[1]
;
Ferreira, Luiz Eduardo N.
[2]
;
Amorim, Klinger S.
[1]
;
Sanchez, Jonny B.
[1]
;
Gercina, Anne Caroline
[1]
;
dos Santos, Victor Augusto B.
[1]
;
Groppo, Francisco C.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, UNICAMP Piracicaba, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Guarulhos, Lab Inflammat & Immunol, Guarulhos, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER; v. 239, JAN 2022. |
| Web of Science Citations: | 0 |
| Abstract | |
This study aimed to evaluate the effects of omega-3 (w3) polyunsaturated fatty acids, in association with aspirin (AA), on the morphology of cytokine release in the temporomandibular joint (TMJ) of rats induced with rheumatoid arthritis (IR) by injecting 100 mu L of complete Freund's adjuvant with bovine type II collagen at the tail base. Thirty-two adult male rats were divided into treatment groups: Sham, treated with 0.9% sodium chloride (NaCl) p.o.; IR-control, treated with 0.9% NaCl p.o.; IR-w3 treated with w3 PUFAS (85 mg/kg/day p.o.); and IR-w3 + AA treated with w3 (85 mg/kg/day p.o.) + AA (20 mg/kg/day i.p.). After maintained treatment for seven days, the animals were euthanized. Bilateral TMJs from each rat were removed and one was subjected to histological immunoassays and enzyme-linked immunosorbent assays to assess interleukin (IL)-113, tumor necrosis factor-a, and IL-10 levels. Data analysis was performed using the Kruskal-Wallis and Dunn tests. In the IR-w3 and IR-w3 + AA groups, the TMJ was greater than in the IR-control group (P < 0.0001). The addition of AA did not improve the effects of w3 (P = 0.0698). Similarly, the addition of AA conferred no additional effects on the cytokine levels (P > 0.05); however, it increased the proteoglycan density, compared with w3 alone. We found that w3 exhibited anti-inflammatory activity in arthritic rats, and the addition of AA increased proteoglycan density, but did not affect cytokine expression. (c) 2021 Elsevier GmbH. All rights reserved. (AU) | |
| FAPESP's process: | 17/17472-3 - The effect of association of aspirin and omega 3 in a CFA-induced inflammatory process in the temporomandibular joint (TMD) of rats: a pilot study |
| Grantee: | Beatriz Hidalgo Ceotto |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |