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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Gut-licensed IFN gamma(+) NK cells drive LAMP1(+)TRAIL(+) anti-inflammatory astrocytes

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Autor(es):
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Sanmarco, Liliana M. [1] ; Wheeler, Michael A. [2, 1] ; Gutierrez-Vazquez, Cristina [1] ; Polonio, Carolina Manganeli [1] ; Linnerbauer, Mathias [1] ; Pinho-Ribeiro, Felipe A. [3] ; Li, Zhaorong [2, 1] ; Giovannoni, Federico [1] ; Batterman, Katelyn V. [4] ; Scalisi, Giulia [1] ; Zandee, Stephanie E. J. [5, 6] ; Heck, Evelyn S. [1] ; Alsuwailm, Moneera [1, 7] ; Rosene, Douglas L. [4] ; Becher, Burkhard [8] ; Chiu, Isaac M. [3] ; Prat, Alexandre [5, 6] ; Quintana, Francisco J. [2, 1]
Número total de Autores: 18
Afiliação do(s) autor(es):
[1] Harvard Med Sch, Brigham & Womens Hosp, Ann Romney Ctr Neurol Dis, Boston, MA 02115 - USA
[2] Broad Inst MIT & Harvard, Cambridge, MA 02142 - USA
[3] Harvard Med Sch, Dept Immunol, Boston, MA 02115 - USA
[4] Boston Univ, Sch Med, Dept Anat & Neurobiol, Boston, MA 02118 - USA
[5] CHUM, Ctr Rech, Neuroimmunol Unit, Montreal, PQ - Canada
[6] Univ Montreal, Fac Med, Dept Neurosci, Montreal, PQ - Canada
[7] King Abdulaziz City Sci & Technol KACST, Ctr Excellence Biomed, Joint Ctr Excellence Program, Riyadh - Saudi Arabia
[8] Univ Zurich, Inst Expt Immunol, Zurich - Switzerland
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: Nature; v. 590, n. 7846, p. 473+, FEB 18 2021.
Citações Web of Science: 32
Resumo

Astrocytes are glial cells that are abundant in the central nervous system (CNS) and that have important homeostatic and disease-promoting functions(1). However, little is known about the homeostatic anti-inflammatory activities of astrocytes and their regulation. Here, using high-throughput flow cytometry screening, single-cell RNA sequencing and CRISPR-Cas9-based cell-specific in vivo genetic perturbations in mice, we identify a subset of astrocytes that expresses the lysosomal protein LAMP1(2) and the death receptor ligand TRAIL(3). LAMP1(+)TRAIL(+) astrocytes limit inflammation in the CNS by inducing T cell apoptosis through TRAIL-DR5 signalling. In homeostatic conditions, the expression of TRAIL in astrocytes is driven by interferon-gamma (IFN gamma) produced by meningeal natural killer (NK) cells, in which IFN gamma expression is modulated by the gut microbiome. TRAIL expression in astrocytes is repressed by molecules produced by T cells and microglia in the context of inflammation. Altogether, we show that LAMP1(+)TRAIL(+) astrocytes limit CNS inflammation by inducing T cell apoptosis, and that this astrocyte subset is maintained by meningeal IFN gamma(+) NK cells that are licensed by the microbiome. (AU)

Processo FAPESP: 19/13731-0 - MicroRNAs, neurodesenvolvimento e resposta immune antiviral: como eles podem estar conectados?
Beneficiário:Carolina Manganeli Polonio
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado