Curcumin-loaded Pluronic (R) F-127 Micelles as a Drug Delivery System for Curcumin-mediated Photodynamic Therapy for Oral Application

Autor(es)

Dantas Lopes dos Santos, Diego ^[1] ; Besegato, Joao Felipe ^[2] ; deMelo, Priscila Borges Gobbo ^[2] ; Oshiro Junior, Joao Augusto ^[3] ; Chorilli, Marlus ^[3] ; Deng, Dongmei ^{[4, 5]} ; Bagnato, Vanderlei Salvador ^[6] ; Rastelli, Alessandra Nara de Souza ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Araraquara, SP - Brazil

^[4] Vrije Univ Amsterdam, Amsterdam - Netherlands

^[5] Univ Amsterdam, Acad Ctr Dent Amsterdam ACTA, Dept Prevent Dent, Amsterdam - Netherlands

^[6] Univ Sao Paulo, Phys Inst Sao Carlos IFSC, Dept Phys & Mat Sci, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 97, n. 5, p. 1072-1088, SEP 2021.
• Citações Web of Science: 3

Resumo

Antimicrobial photodynamic therapy (aPDT) is promising for oral decontamination. Curcumin has been used as photosensitizer; however, the hydrophobic properties can negatively affect aPDT. This study evaluated the aPDT efficacy using Cur-loaded Pluronic (R) F-127 micelles against Streptococcus mutans and Candida albicans biofilms. Micelles characterization was performed by zeta potential, dynamic light scattering, transmission electron microscopy, absorption and fluorescence spectroscopy. Cur concentrations, cell viability by CFU mL(-1) and confocal microscopy were determined. Data were analyzed by parametric and nonparametric tests under 5%. Cur-loaded Pluronic (R) F-127 exhibited spherical shape, suitable particle size (<= 100 nm), adequate polydispersity index, best stability, lower photodegradation and auto-aggregation compared to unloaded-Cur. Both microorganisms were sensitive to Cur-loaded Pluronic (R) F-127 micelles aPDT, with minimum inhibitory concentration (MIC) of 270 mu M and 2.1093 mu M for S. mutans and C. albicans suspended culture, respectively. Cur-loaded Pluronic (R) F-127 aPDT exhibited antibacterial/antifungal effect against the biofilms (similar to 3 log(10) reduction; P <= 0.05); however, similar to unloaded (P >= 0.05). Confocal images confirmed these results. Cur-loaded Pluronic (R) F-127 micelles exhibited good photochemical properties and may be a viable alternative to deliver Cur and to improve aPDT effect during the treatment of dental caries. Moreover, Pluronic (R) micelles can enhance the solubility, stability, permeability and control the release of Cur. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/17839-4 - Inativação fotodinâmica utilizando-se curcumina conjugada a pluronic®F-127 sobre biofilme de Streptococcus mutans e Candida albicans
• Beneficiário: Diego Dantas Lopes dos Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado