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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Curcumin-loaded Pluronic (R) F-127 Micelles as a Drug Delivery System for Curcumin-mediated Photodynamic Therapy for Oral Application

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Author(s):
Dantas Lopes dos Santos, Diego [1] ; Besegato, Joao Felipe [2] ; deMelo, Priscila Borges Gobbo [2] ; Oshiro Junior, Joao Augusto [3] ; Chorilli, Marlus [3] ; Deng, Dongmei [4, 5] ; Bagnato, Vanderlei Salvador [6] ; Rastelli, Alessandra Nara de Souza [2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Araraquara, SP - Brazil
[4] Vrije Univ Amsterdam, Amsterdam - Netherlands
[5] Univ Amsterdam, Acad Ctr Dent Amsterdam ACTA, Dept Prevent Dent, Amsterdam - Netherlands
[6] Univ Sao Paulo, Phys Inst Sao Carlos IFSC, Dept Phys & Mat Sci, Sao Carlos, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Photochemistry and Photobiology; v. 97, n. 5, p. 1072-1088, SEP 2021.
Web of Science Citations: 3
Abstract

Antimicrobial photodynamic therapy (aPDT) is promising for oral decontamination. Curcumin has been used as photosensitizer; however, the hydrophobic properties can negatively affect aPDT. This study evaluated the aPDT efficacy using Cur-loaded Pluronic (R) F-127 micelles against Streptococcus mutans and Candida albicans biofilms. Micelles characterization was performed by zeta potential, dynamic light scattering, transmission electron microscopy, absorption and fluorescence spectroscopy. Cur concentrations, cell viability by CFU mL(-1) and confocal microscopy were determined. Data were analyzed by parametric and nonparametric tests under 5%. Cur-loaded Pluronic (R) F-127 exhibited spherical shape, suitable particle size (<= 100 nm), adequate polydispersity index, best stability, lower photodegradation and auto-aggregation compared to unloaded-Cur. Both microorganisms were sensitive to Cur-loaded Pluronic (R) F-127 micelles aPDT, with minimum inhibitory concentration (MIC) of 270 mu M and 2.1093 mu M for S. mutans and C. albicans suspended culture, respectively. Cur-loaded Pluronic (R) F-127 aPDT exhibited antibacterial/antifungal effect against the biofilms (similar to 3 log(10) reduction; P <= 0.05); however, similar to unloaded (P >= 0.05). Confocal images confirmed these results. Cur-loaded Pluronic (R) F-127 micelles exhibited good photochemical properties and may be a viable alternative to deliver Cur and to improve aPDT effect during the treatment of dental caries. Moreover, Pluronic (R) micelles can enhance the solubility, stability, permeability and control the release of Cur. (AU)

FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 17/17839-4 - Photodinamic inactivation using curcumin conjugated to pluronic ®F-127 on Streptococcus mutans and Candida albicans biofilm
Grantee:Diego Dantas Lopes dos Santos
Support Opportunities: Scholarships in Brazil - Master