Whole-genome sequencing reveals high genetic diversity of Streptococcus uberis isolated from cows with mastitis

Autor(es)

Silva, Nathalia Cristina Cirone ^{[1, 2]} ; Yang, Yongqiang ^{[3, 4]} ; Rodrigues, Marjory Xavier ^[1] ; Tomazi, Tiago ^[1] ; Bicalho, Rodrigo Carvalho ^[1]

Afiliação do(s) autor(es)

^[1] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 - USA

^[2] Univ Campinas UNICAMP, Fac Food Engn FEA, Dept Food Sci & Nutr, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[3] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Microbiol, Guangzhou 510080 - Peoples R China

^[4] Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Guangzhou 510006 - Peoples R China

• Tipo de documento: Artigo Científico
• Fonte: BMC Veterinary Research; v. 17, n. 1 OCT 7 2021.
• Citações Web of Science: 0

Resumo

Background Bovine mastitis is an important cause of economic loss in dairy farms. Streptococcus uberis is among the most frequently isolated bacterial species isolated from cows with mastitis. The aim of this study was to perform an in-depth genetic assessment of S. uberis strains isolated from bovine clinical mastitis (CM) and to perform a phylogenetic analysis to represent the evolutionary relationship among S. uberis sequences. Results A total of 159 isolates was genetically characterized using whole genome sequencing. According to the virulence determinants, all strains harbored the hasC, leuS, perR, purH, and purN virulence genes. Thirty-four resistance genes were identified in at least one strain. In terms of acquired genes, we observed that 152 (95.6 %) strains had a resistance gene to lincosamine (lnuD), 48 (30.2 %) to tetracycline (tetM), 4 (2.51 %) to tobramicine (ant6), and 1 to lincosamide (lsa(E)). MLST detected the Sequence Type (ST)797 (n = 23), while 85.5 % of the strains did not match to known STs. Conclusions Then, eleven distinct ST were identified after we submitted the new alleles to assign new STs. The other prevalent STs observed were ST1215 (n = 58), ST1219 (n = 35), and ST1213 (n = 15). And it was not possible to identify the MLST of four strains. Phylogenetic lineages indicated a high genomic diversity of S. uberis in our collection, confirming that most strains isolated from bovine mastitis have different reservoirs, typical of environmental pathogens. (AU)

• Processo FAPESP: 18/24191-3 - Investigação genômica de Staphylococcus coagulase negativa isolados de vacas com mastite
• Beneficiário: Nathalia Cristina Cirone Silva
• Modalidade de apoio: Bolsas no Exterior - Pesquisa