Neuromotor activity inhibition in zebrafish early-life stages after exposure to environmental relevant concentrations of caffeine

Autor(es)

de Farias, Natalia Oliveira ^{[1, 2, 3]} ; Andrade, Thayres de Sousa ^[4] ; Santos, Viviani Lara ^[1] ; Galvino, Pedro ^[1] ; Suares-Rocha, Paula ^[2] ; Domingues, Ines ^{[5, 6]} ; Grisolia, Cesar Koppe ^[1] ; Oliveira, Rhaul ^[2]

Afiliação do(s) autor(es)

^[1] Univ Brasilia, Inst Ciencias Biol, Dept Genet & Morfol, Lab Genet Toxicol, Brasilia, DF - Brazil

^[2] Univ Estadual Campinas, Fac Tecnol, UNICAMP, Campus Limeira 1, BR-13484332 Limeira, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, IB UNICAMP, Programa Posgrad Biol Anim, Campinas, SP - Brazil

^[4] Univ Fed Ceara, Dept Engn Ambiental, UFC, Crateus, Ceara - Brazil

^[5] Univ Aveiro, Dept Biol, Campus Univ Santiago, Aveiro - Portugal

^[6] Univ Aveiro, CESAM, Campus Univ Santiago, Aveiro - Portugal

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING; v. 56, n. 12 OCT 2021.
• Citações Web of Science: 0

Resumo

Caffeine (CAF), a neuroactive compound, has been found in surface waters at concentrations ranging from few nanograms up to micrograms and may induce adverse effects in aquatic vertebrates. Thus, the aim of this study was to evaluate the potential of CAF in affecting fish early-life stages in a wide concentration range, including occurring levels in surface waters. Specimens of zebrafish in early-life stages were exposed to CAF for 168 h and survival, developmental alterations, locomotor activity and acetylcholinesterase activity were evaluated. CAF induced mortality in embryos unable to hatch or in larvae after hatching (LC50 - 168 h = 283.2 mg/L). Tail deformities were observed in organisms exposed to concentrations >= 40 mg/L, while edemas were found at concentrations of 100 mg/L. CAF also decreased the total swimming time and distance moved of exposed organisms (LOEC = 0.0006 mg/L). Locomotor inhibition may be associated with an acetylcholinesterase inhibition observed at concentration >= 0.0088 mg/L. Therefore, the hazard of CAF for fish populations deserves further attention since unexpected effects on neuro-behavioral parameters occurs at concentrations often detected in natural aquatic ecosystems. (AU)

• Processo FAPESP: 18/03108-0 - O papel do teste com embrião de Peixe-Zebra na avaliação integrada da qualidade da água em áreas de plantio de cana-de-açúcar
• Beneficiário: Rhaul de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado