Imbalance between the circulating endothelium-derived apoptotic microparticles and the endothelial colony-forming units of progenitor cells in patients undergoing diagnostic coronary angiography

Autor(es)

Pimentel Mota, Isabela Cardoso ^[1] ; Souza, Livia Victorino ^[1] ; Parizotto, Giovanna Pachele ^[1] ; Thomazini, Fernanda ^[2] ; Henao Agudelo, Juan Sebastian ^{[1, 3]} ; Almeida, Danilo Candido ^[1] ; da Costa Junior, Jose Ribamar ^[4] ; Franco, Maria do Carmo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Sch Med, Nephrol Div, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Sch Med, Program Translat Med, Sao Paulo - Brazil

^[3] Fac Hlth Sci, Cent Unit Valle Del Cauca, Valle Del Cauca, Tulua - Colombia

^[4] Dante Pazzanese Inst Cardiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ADVANCES IN MEDICAL SCIENCES; v. 66, n. 2, p. 396-402, SEP 2021.
• Citações Web of Science: 0

Resumo

Purpose: The involvement of the circulating endothelium-derived microparticles (EMPs) and the endothelial progenitor cells (EPCs) has been shown in the pathogenesis of coronary artery disease (CAD). The current study aimed to explore whether the Friesinger index is associated with the levels of the apoptotic CD144+/CD31+/ annexin V+ EMPs and the number of endothelial colony-forming units of progenitor cells in patients undergoing coronary angiography. Patients and methods: Fifty-seven patients with a median age of 62 years (range: 48-84 years) were enrolled. Quantification of the apoptotic CD144+/CD31+/annexin V+ EMPs was performed by flow cytometry. The number of endothelial colony-forming units defined by CFU-Hill was assessed by cell culture. Results: There was a positive correlation between the Friesinger index and the circulating levels of the apoptotic CD144+/CD31+/annexin V+ EMPs (rho=0.817, p<0.001), whereas a negative correlation was found with the number of CFU-Hill (rho = - 0.649, p<0.001). Multivariable logistic analysis showed that the risk of having moderate/severe CAD was five times greater among male patients (OR:5.32; 95% CI: 1.19 - 16.33; p=0.038) and almost one and a half times higher among those with a higher level of apoptotic CD144+/CD31+/annexin V+ EMPs (OR:1.74; 95% CI: 1.23 - 2.28; p=0.001). Finally, the circulating levels of apoptotic EMPs labelled for CD144+/CD31+/annexin V+ presented a good discrimination of moderate/severe CAD, with an AUC of 0.85 (95% CI = 0.74 - 0.96; p< 0.001). Conclusions: Moderate or severe CAD is associated with increased levels of apoptotic EMPs and reduced EPC colony-forming capacity, increasing the occurrence of endothelial injuries. (AU)

• Processo FAPESP: 17/08361-3 - Avaliação das interações do exercício físico recreativo, peso ao nascer, níveis pressóricos e os componentes do sistema renina-angiotensina-calicreína-cininas em crianças
• Beneficiário: Livia Victorino de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/04013-3 - Quantificação da população de micropartículas de origem endotelial circulantes e análise do seu perfil de miRNAs: avaliação do impacto sobre a inter-relação entre o peso ao nascer, níveis pressóricos e função endotelial em crianças de 6 a 12 anos
• Beneficiário: Maria Do Carmo Pinho Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular