Magnetic 3D cell culture: State of the art and current advances

Autor(es)

Caleffi, Juliana Trindade ^[1] ; Esgoti Aal, Mirian Carolini ^[1] ; Manacorda Gallindo, Helena de Oliveira ^[1] ; Caxali, Gabriel Henrique ^[1] ; Crulhas, Bruno Pereira ^[2] ; Ribeiro, Amanda Oliveira ^[1] ; Souza, Glauco R. ^[3] ; Delella, Flavia Karina ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Botucatu, SP - Brazil

^[2] Remedy Biol, Dublin - Ireland

^[3] Univ Texas Hlth Sci Ctr Houston, Houston, TX 77030 - USA

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 286, DEC 1 2021.
• Citações Web of Science: 0

Resumo

Cell culture is an important tool for the understanding of cell biology and behavior. In vitro cultivation has been increasingly indispensable for biomedical, pharmaceutical, and biotechnology research. Nevertheless, with the demand for in vitro experimentation strategies more representative of in vivo conditions, tridimensional (3D) cell culture models have been successfully developed. Although these 3D models are efficient and address critical questions from different research areas, there are considerable differences between the existing techniques regarding both elaboration and cost. In light of this, this review describes the construction of 3D spheroids using magnetization while bringing the most recent updates in this field. Magnetic 3D cell culture consists of magnetizing cells using an assembly of gold and iron oxide nanoparticles cross-linked with poly-L-lysine nanoparticles. Then, 3D culture formation in special plates with the assistance of magnets for levitation or bioprinting. Here, we discuss magnetic 3D cell culture advancements, including tumor microenvironment, tissue reconstruction, blood vessel engineering, toxicology, cytotoxicity, and 3D culture of cardiomyocytes, bronchial and pancreatic cells. (AU)

• Processo FAPESP: 19/01869-7 - Efeito isolado ou sinérgico do ácido anacárdico em células neoplásicas malignas
• Beneficiário: Flávia Karina Delella
• Modalidade de apoio: Auxílio à Pesquisa - Regular