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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Magnetic 3D cell culture: State of the art and current advances

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Author(s):
Caleffi, Juliana Trindade [1] ; Esgoti Aal, Mirian Carolini [1] ; Manacorda Gallindo, Helena de Oliveira [1] ; Caxali, Gabriel Henrique [1] ; Crulhas, Bruno Pereira [2] ; Ribeiro, Amanda Oliveira [1] ; Souza, Glauco R. [3] ; Delella, Flavia Karina [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Botucatu, SP - Brazil
[2] Remedy Biol, Dublin - Ireland
[3] Univ Texas Hlth Sci Ctr Houston, Houston, TX 77030 - USA
Total Affiliations: 3
Document type: Journal article
Source: Life Sciences; v. 286, DEC 1 2021.
Web of Science Citations: 0
Abstract

Cell culture is an important tool for the understanding of cell biology and behavior. In vitro cultivation has been increasingly indispensable for biomedical, pharmaceutical, and biotechnology research. Nevertheless, with the demand for in vitro experimentation strategies more representative of in vivo conditions, tridimensional (3D) cell culture models have been successfully developed. Although these 3D models are efficient and address critical questions from different research areas, there are considerable differences between the existing techniques regarding both elaboration and cost. In light of this, this review describes the construction of 3D spheroids using magnetization while bringing the most recent updates in this field. Magnetic 3D cell culture consists of magnetizing cells using an assembly of gold and iron oxide nanoparticles cross-linked with poly-L-lysine nanoparticles. Then, 3D culture formation in special plates with the assistance of magnets for levitation or bioprinting. Here, we discuss magnetic 3D cell culture advancements, including tumor microenvironment, tissue reconstruction, blood vessel engineering, toxicology, cytotoxicity, and 3D culture of cardiomyocytes, bronchial and pancreatic cells. (AU)

FAPESP's process: 19/01869-7 - Isolated or synergistic effect of anacardic acid on malignant neoplastic cells
Grantee:Flávia Karina Delella
Support Opportunities: Regular Research Grants