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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Functional properties and potential application of ethanol tolerant beta-glucosidases from Pichia ofunaensis and Trichosporon multisporum yeasts

Texto completo
Autor(es):
Vaz, Jaqueline Elaine [1] ; Rabelo, Lacan [1] ; Zaiter, Mohammed Anas [1] ; Pereira, Waldir Eduardo Simioni [1] ; Metzker, Gustavo [1] ; Boscolo, Mauricio [1] ; da Silva, Roberto [1] ; Gomes, Eleni [1] ; da Silva, Ronivaldo Rodrigues [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, 2265 Jd Nazareth, Ibilce Unesp, Sao Jose do Rio Preto, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: 3 BIOTECH; v. 11, n. 11 NOV 2021.
Citações Web of Science: 0
Resumo

beta-Glucosidases have been extensively investigated to integrate the enzyme complex for cellulose fiber saccharification and for improving the aroma of wine. To produce these enzymes, greater attention has been given to filamentous fungi and bacteria, and few investigations have targeted the potential applications of enzymes secreted by yeasts. Addressing this issue, in this study, beta-glucosidases were produced by the Pichia ofunaensis and Trichosporon multisporum yeasts, via solid state fermentation with wheat bran as a substrate. When using p-Nitrophenyl beta-d-glucopyranoside (pNPG) as an enzyme substrate, maximum beta-glucosidase activities were detected at pH 5.5-6.0 and 50-60 degrees C for P. ofunaensis, and pH 5-6 and 55 degrees C for T. multisporum. Both enzymes were able to hydrolyze cellobiose and exhibited stability over a wide range of pH (3.5-9.0) for 24 h at 4 degrees C, thermostability up to 50 degrees C for 1 h and tolerance to 10 mM phenolic compounds. Negative modulation on enzyme activity was observed in the presence of Cu2+, Fe3+, Zn2+, Al3+ and Hg2+, while both beta-glucosidases were tolerant to 30% methanol, isopropanol and acetone. In the presence of ethanol and glucose, enzymes from P. ofunaensis were the more active and stable of the two. These enzymes, especially the P. ofunaensis beta-glucosidases, could be tested in enology for improving the aroma of wine and for integrating a cellulolytic complex to produce 2G ethanol. (AU)

Processo FAPESP: 17/06066-4 - Leveduras nativas assimiladores de xilose e arabinose: estudo da assimilação e metabolização das pentoses e aproveitamento desses açúcares oriundos da hidrólise da biomassa lignocelulósica para obtenção de bioprodutos
Beneficiário:Eleni Gomes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/06399-3 - Estudo do sistema de transporte membranar e assimilação de L-arabinose e xilose pelas leveduras Aureobasidium leucospermi LB86, Candida oleophila G10.1, Issatchenkia terricola G20 e Pichia guilliermondii G1.2
Beneficiário:Ronivaldo Rodrigues da Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/07036-4 - Avaliação das condições de cultivo para assimilação de xilose e secreção de enzimas e peptídeos por leveduras isoladas do ambiente
Beneficiário:Jaqueline Elaine Vaz
Linha de fomento: Bolsas no Brasil - Mestrado