Functional properties and potential application of ethanol tolerant beta-glucosidases from Pichia ofunaensis and Trichosporon multisporum yeasts

Author(s)

Vaz, Jaqueline Elaine ^[1] ; Rabelo, Lacan ^[1] ; Zaiter, Mohammed Anas ^[1] ; Pereira, Waldir Eduardo Simioni ^[1] ; Metzker, Gustavo ^[1] ; Boscolo, Mauricio ^[1] ; da Silva, Roberto ^[1] ; Gomes, Eleni ^[1] ; da Silva, Ronivaldo Rodrigues ^[1]

Affiliation

^[1] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, 2265 Jd Nazareth, Ibilce Unesp, Sao Jose do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: 3 BIOTECH; v. 11, n. 11 NOV 2021.
• Web of Science Citations: 0

Abstract

beta-Glucosidases have been extensively investigated to integrate the enzyme complex for cellulose fiber saccharification and for improving the aroma of wine. To produce these enzymes, greater attention has been given to filamentous fungi and bacteria, and few investigations have targeted the potential applications of enzymes secreted by yeasts. Addressing this issue, in this study, beta-glucosidases were produced by the Pichia ofunaensis and Trichosporon multisporum yeasts, via solid state fermentation with wheat bran as a substrate. When using p-Nitrophenyl beta-d-glucopyranoside (pNPG) as an enzyme substrate, maximum beta-glucosidase activities were detected at pH 5.5-6.0 and 50-60 degrees C for P. ofunaensis, and pH 5-6 and 55 degrees C for T. multisporum. Both enzymes were able to hydrolyze cellobiose and exhibited stability over a wide range of pH (3.5-9.0) for 24 h at 4 degrees C, thermostability up to 50 degrees C for 1 h and tolerance to 10 mM phenolic compounds. Negative modulation on enzyme activity was observed in the presence of Cu2+, Fe3+, Zn2+, Al3+ and Hg2+, while both beta-glucosidases were tolerant to 30% methanol, isopropanol and acetone. In the presence of ethanol and glucose, enzymes from P. ofunaensis were the more active and stable of the two. These enzymes, especially the P. ofunaensis beta-glucosidases, could be tested in enology for improving the aroma of wine and for integrating a cellulolytic complex to produce 2G ethanol. (AU)

• FAPESP's process: 17/06066-4 - Native xylose and arabinose yeasts-assimilating: study of the assimilation and metabolization of the pentoses and use of these sugars from lignocellulosic biomass for obtainment bio-products
• Grantee: Eleni Gomes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/06399-3 - Investigating the membrane transport and assimilation of L-arabinose and xylose by the yeasts leucospermi LB86, Candida oleophila G10.1, Issachenkia terricola G20 and Pichia guilliermondii G1.2
• Grantee: Ronivaldo Rodrigues da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/07036-4 - Evaluation of culture conditions for xylose assimilation and secretion of enzymes and peptides by yeasts isolated from the environment
• Grantee: Jaqueline Elaine Vaz
• Support Opportunities: Scholarships in Brazil - Master