Effectiveness of a new rutin Cu(II) complex in the prevention of lipid peroxidation and hepatotoxicity in hypercholesterolemic rats

Autor(es)

de Oliveira, Daniel A. F. ^[1] ; Diniz, Susana N. ^[1] ; Pereira, Regina M. S. ^[1] ; Goncalves, Ivair D. ^[1] ; Renno, Andre L. ^{[2, 3]} ; Gorjao, Renata ^[4] ; Vieira, Eduardo G. ^[5] ; Ferreira, Ana M. C. ^[5] ; Okuyama, Cristina E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Anhanguera Sao Paulo UNIAN, Sao Paulo - Brazil

^[2] Ctr Univ Jaguariuna UNIFAJ, Fac Pharm, Jaguariuna - Brazil

^[3] Fac Sao Leopoldo Mandic SLM, Fac Med, Campinas - Brazil

^[4] Univ Cruzeiro do Sul UNICSUL, Program Interdisciplinary Postgrad Hlth Sci, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Chem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FOOD BIOCHEMISTRY; NOV 2021.
• Citações Web of Science: 0

Resumo

A new rutin copper(II) complex (R-Cu2) was prepared and characterized by spectroscopic methods and elemental analysis. The effects of rutin and R-Cu2 were evaluated on the prevention of hypercholesterolemia in animals feed with high-cholesterol diet (HCD) for 8 weeks. The animals (n = 5) were neither fed with HCD nor treated (control group), or were treated with vehicle, 10 mg/kg simvastatin, rutin (16 and 160 mu mol/kg), and R-Cu2 (16 and 160 mu mol/kg) administered orally. Total cholesterol (TC) levels were significantly increased (p < .01) in all HCD groups. In rutin and R-Cu2 groups, it was observed a discrete, but not significant, TC and LDL-induced increase inhibition compared with vehicle-treated group. R-Cu2 treatment significantly decreased (p < .05) plasma triglycerides compared with the vehicle-treated group. All groups receiving treatments maintained the malondialdehyde at normal levels. Serum NO levels were reduced in animals treated with rutin and R-Cu2 compared with the vehicle-treated group. In addition, the results also showed that the groups treated with rutin and R-Cu2 reduced significantly (p < .01), the number of neutrophils and prevented histological changes in all evaluated liver zones. R-Cu2 group maintained the ALT, AST, and ALP enzymes at normal levels. Thus, the effects of R-Cu2 in modulating inflammation and protecting liver damage were confirmed. Practical applications Rutin, a plant-derived flavonoid, is one of phenolic compounds well known as a nutraceutical due to its antioxidant and anti-inflammatory properties. Findings of this study demonstrate the effects of both rutin and R-Cu2 in modulating inflammation and protecting liver damage in hypercholesterolemic rats. However, some effects analyzed became more evident in R-Cu2. Thereby, it was shown that the synthesis of a new flavonoid compound (R-Cu2) could be applied as a nutraceutical benefit option to prevent hypercholesterolemia condition. (AU)

• Processo FAPESP: 09/00344-6 - Avaliação das propriedades farmacológicas de flavonóides e derivados contendo metais de transição.
• Beneficiário: Cristina Eunice Okuyama Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/14159-9 - Síntese, caracterização e estudos da fluorescência de compostos cumarínicos contendo metais de transição da primeira série: avaliação do seu potencial antiparasitário
• Beneficiário: Regina Mara Silva Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular