otential of thaxtomin A for the control of the Asian soybean rus

Autor(es)

De Paula, Samuel ^[1] ; Durigan Dalio, Ronaldo Jose ^[2] ; Maximo, Heros Jose ^[2] ; Pino, Lilian Ellen ^[2] ; Amorim, Deoclecio Jardim ^[3] ; Rodrigues Da Paz, Suzani Maria ^[1] ; Ribeiro Brandao, Dayson Fernando ^[4] ; Borges Demetrio, Clarice Garcia ^[3] ; Pascholati, Sergio Florentino ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Plant Pathol & Nematol, ESALQ, BR-13418900 Piracicaba - Brazil

^[2] IdeeLab Biotecnol, BR-13414157 Piracicaba - Brazil

^[3] Univ Sao Paulo, Dept Exact Sci, ESALQ, BR-13418900 Piracicaba - Brazil

^[4] Stoller Brazil, Dept Res & Dev, BR-13091908 Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE; v. 44, n. 1 NOV 2021.
• Citações Web of Science: 0

Resumo

Several strategies are required to achieve satisfactory control of Asian soybean rust (ASR) caused by the fungus Phakopsora pachyrhizi. The use of resistance inducers represents a helpful alternative for management of this disease, since they can present good results when included in an integrated ASR management programme. The aim of this study was to investigate the effect of the partially purified phytotoxin thaxtomin A (PPT) in the induction of resistance to P. pachyrhizi in soybean plants and the direct effect on urediniospore germination. Detached soybean leaves were used to test the induction of resistance. They were treated with PPT 72 h before inoculation with the fungus. The disease severity was evaluated after 12 days. For gene expression analysis, leaf samples were collected 24 h after the application of the inducer. The results showed that PPT had a direct effect on spore germination. A concentration of 100 mu g PPT mL(-1) inhibited the germination of P. pachyrhizi urediniospores by 64.7%. The phytotoxin was also able to reduce the severity of ASR in detached leaves, possibly through the activation of defence mechanisms related to the jasmonate/ethylene pathways. The application of PPT resulted in upregulation of the expression of the ACC gene, the precursor of ethylene biosynthesis, which is involved in one of the three signalling pathways of defence in plants. Thus, these results suggest that the partially purified thaxtomin A is a potential agent for the control of ASR. (AU)

• Processo FAPESP: 19/23030-9 - Desenvolvimento de produto biotecnológico contendo o fungo Memnoniella levispora para uso na agricultura
• Beneficiário: Héros José Maximo
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE