Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

Autor(es)

Yassitepe, Juliana Erika de Carvalho Teixeira ^{[1, 2, 3]} ; da Silva, Viviane Cristina Heinzen ^{[1, 3]} ; Hernandes-Lopes, Jose ^{[1, 2, 3]} ; Dante, Ricardo Augusto ^{[1, 2, 3]} ; Gerhardt, Isabel Rodrigues ^{[1, 2, 3]} ; Fernandes, Fernanda Rausch ^{[1, 2, 3]} ; da Silva, Priscila Alves ^{[1, 3]} ; Vieira, Leticia Rios ^{[1, 3]} ; Bonatti, Vanessa ^{[1, 3]} ; Arruda, Paulo ^{[1, 3, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas - Brazil

^[2] EMBRAPA Informat Agr, Campinas - Brazil

^[3] Univ Estadual Campinas, Genom Climate Change Res Ctr GCCRC, Campinas - Brazil

^[4] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, Campinas - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 12, OCT 14 2021.
• Citações Web of Science: 0

Resumo

Over the past decades, advances in plant biotechnology have allowed the development of genetically modified maize varieties that have significantly impacted agricultural management and improved the grain yield worldwide. To date, genetically modified varieties represent 30% of the world's maize cultivated area and incorporate traits such as herbicide, insect and disease resistance, abiotic stress tolerance, high yield, and improved nutritional quality. Maize transformation, which is a prerequisite for genetically modified maize development, is no longer a major bottleneck. Protocols using morphogenic regulators have evolved significantly towards increasing transformation frequency and genotype independence. Emerging technologies using either stable or transient expression and tissue culture-independent methods, such as direct genome editing using RNA-guided endonuclease system as an in vivo desired-target mutator, simultaneous double haploid production and editing/haploid-inducer-mediated genome editing, and pollen transformation, are expected to lead significant progress in maize biotechnology. This review summarises the significant advances in maize transformation protocols, technologies, and applications and discusses the current status, including a pipeline for trait development and regulatory issues related to current and future genetically modified and genetically edited maize varieties.</p> (AU)

• Processo FAPESP: 20/09007-1 - Centro de Pesquisa em Genômica Aplicada às Mudanças Climáticas
• Beneficiário: Vanessa Bonatti
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 18/06442-9 - Descoberta de novas quinases associadas à resposta ao estresse hídrico em milho
• Beneficiário: Viviane Cristina Heinzen da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/23218-0 - Centro de Pesquisa em Genômica Aplicada às Mudanças Climáticas
• Beneficiário: Edi Lúcia Sartorato
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
• Processo FAPESP: 20/10677-1 - Transformação genética de milho mediada por Agrobacterium tumefaciens para obtenção de resistência a estresses abióticos
• Beneficiário: Letícia Rios Vieira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado