New prospects and problems in sample preparation methods for microbiome analysis

Autor(es)

Belinato, Joao Raul ^{[1, 2]} ; Acquaro, Jr., Vinicius Ricardo ^[3] ; Dias Cappelini, Luciana Teresa ^[4] ; Augusto, Fabio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Natl Inst Sci & Technol Bioanalyt INCTBio, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Apex Sci Analyt Consulting, Ave Marechal Rondon 2148, BR-13070175 Campinas, SP - Brazil

^[3] Scientia Consultoria Cient, BR-03362000 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: TRAC-TRENDS IN ANALYTICAL CHEMISTRY; v. 143, OCT 2021.
• Citações Web of Science: 0

Resumo

Since the discovery of the first bioactive compounds from microorganisms, interest in these systems has gradually increased. Microorganisms have been considered an essential source for biomolecules with several applications such as new drugs, biopesticides, etc. One of the significant challenges in microorganism exploration is sample preparation prior to instrumental analysis, which involves extraction and clean-up processes. So far, conventional sample preparation techniques such as solid phase extraction (SPE), liquid-liquid extraction (LLE), and solvent extraction (SE) are still widespread in microbiological research. Although they present several disadvantages compared to modern methods, they continue being used. Thus, microextraction techniques in this field can be employed to overcome several limitations of conventional methods. This review presents the advantages of microextraction as well as examples of its application to encourage the use of microextraction techniques. In addition to recent applications, basic theory that indicates which kind of strategies can favor a more profound knowledge of these highly complex systems is also discussed. Indeed, appropriate sample preparation techniques can support the understanding of these systems and promote the discovery of new molecules. (C) 2021 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 18/25729-7 - Revestimentos SPME biocompatíveis de alto rendimento para análises metabolômicas
• Beneficiário: Joao Raul Belinato de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 17/08038-8 - Busca de potenciais produtores de peptídeos não ribossômicos (NRPS) na compostagem da Fundação Parque Zoológico de São Paulo, utilizando iChip e Prism
• Beneficiário: Luciana Teresa Dias Cappelini
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 18/03956-1 - Desenvolvimento e aplicação do novo dispositivo SPME - "Coated Blade Spray"
• Beneficiário: Vinicius Ricardo Acquaro Junior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/20547-2 - Caracterização do metaboloma de microorganismos anti-fitopatógenos por Cromatografia Gasosa Multidimensional e Espectrometria de Massas Simples e em Tandem(GC×GC-QMS e GC×GC-MS/MS)
• Beneficiário: Joao Raul Belinato de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07037-0 - Avaliação química, bioquímica e proteogenômica do processo de compostagem na Fundação Parque Zoológico de São Paulo (FPZSP).
• Beneficiário: Luciana Teresa Dias Cappelini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado