New prospects and problems in sample preparation methods for microbiome analysis

Author(s)

Belinato, Joao Raul ^{[1, 2]} ; Acquaro, Jr., Vinicius Ricardo ^[3] ; Dias Cappelini, Luciana Teresa ^[4] ; Augusto, Fabio ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Natl Inst Sci & Technol Bioanalyt INCTBio, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Apex Sci Analyt Consulting, Ave Marechal Rondon 2148, BR-13070175 Campinas, SP - Brazil

^[3] Scientia Consultoria Cient, BR-03362000 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP - Brazil

• Document type: Review article
• Source: TRAC-TRENDS IN ANALYTICAL CHEMISTRY; v. 143, OCT 2021.
• Web of Science Citations: 0

Abstract

Since the discovery of the first bioactive compounds from microorganisms, interest in these systems has gradually increased. Microorganisms have been considered an essential source for biomolecules with several applications such as new drugs, biopesticides, etc. One of the significant challenges in microorganism exploration is sample preparation prior to instrumental analysis, which involves extraction and clean-up processes. So far, conventional sample preparation techniques such as solid phase extraction (SPE), liquid-liquid extraction (LLE), and solvent extraction (SE) are still widespread in microbiological research. Although they present several disadvantages compared to modern methods, they continue being used. Thus, microextraction techniques in this field can be employed to overcome several limitations of conventional methods. This review presents the advantages of microextraction as well as examples of its application to encourage the use of microextraction techniques. In addition to recent applications, basic theory that indicates which kind of strategies can favor a more profound knowledge of these highly complex systems is also discussed. Indeed, appropriate sample preparation techniques can support the understanding of these systems and promote the discovery of new molecules. (C) 2021 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 18/25729-7 - High throughput biocompatible SPME coatings for metabolomic analyses
• Grantee: Joao Raul Belinato de Souza
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/08038-8 - Search of potential non-ribosomal peptide producers (NRPS) in the compost from the foundation Parque Zoológico de São Paulo using iChip and Prism
• Grantee: Luciana Teresa Dias Cappelini
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 18/03956-1 - Development and application of the novelty SPME device - Coated Blade Spray
• Grantee: Vinicius Ricardo Acquaro Junior
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/20547-2 - Characterization of the metabolome of anti-phytopatogen microorganisms by Multidimensional Gas Chromatography and Simple / Tandem Mass Spectrometry (GC×GC-QMS and GC×GC-MS/MS)
• Grantee: Joao Raul Belinato de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/07037-0 - Chemical and biological assessment of composting produced at the Fundação Parque Zoológico de São Paulo (FPZSP).
• Grantee: Luciana Teresa Dias Cappelini
• Support Opportunities: Scholarships in Brazil - Post-Doctoral