Nano-Infrared Imaging of Primary Neurons

Autor(es):Mostrar menos -

Freitas, Raul O. ^[1] ; Cernescu, Adrian ^[2] ; Engdahl, Anders ^[3] ; Paulus, Agnes ^{[3, 4]} ; Levandoski, Joao E. ^[1] ; Martinsson, Isak ^[5] ; Hebisch, Elke ^[6] ; Sandt, Christophe ^[7] ; Gouras, Gunnar Keppler ^[5] ; Prinz, Christelle N. ^[6] ; Deierborg, Tomas ; Borondics, Ferenc ^[7] ; Klementieva, Oxana ^[3]

Afiliação do(s) autor(es)

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil

^[2] Attocube Syst AG, Eglfinger Weg 2, D-85540 Munich - Germany

^[3] Lund Univ, Dept Expt Med Sci, Med Microspectroscopy, S-22180 Lund - Sweden

^[4] Lund Univ, Dept Expt Med Sci, Expt Neuroinflammat Lab, S-22180 Lund - Sweden

^[5] Lund Univ, Dept Expt Med Sci, Expt Dementia Res, S-22180 Lund - Sweden

^[6] Lund Univ, Div Solid State Phys & NanoLund, S-22100 Lund - Sweden

^[7] Synchrotron SOLEIL, F-91192 Gif Sur Yvette - France

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 10, n. 10 OCT 2021.
• Citações Web of Science: 0

Resumo

Alzheimer's disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-beta (A beta) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, A beta proteins undergo structural changes to form beta-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid beta-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM) to study amyloid structures at the nanoscale, in individual neurons. Specifically, we show that in well-validated systems, s-SNOM can detect amyloid beta-sheet structures with nanometer spatial resolution in individual neurons. This is a proof-of-concept study to demonstrate that s-SNOM can be used to detect A beta-sheet structures on cell surfaces at the nanoscale. Furthermore, this study is intended to raise neurobiologists' awareness of the potential of s-SNOM as a tool for analyzing amyloid beta-sheet structures at the nanoscale in neurons without the need for immunolabeling.</p> (AU)

• Processo FAPESP: 19/14017-9 - De ondas de éxcitons a THz-fônons: a nano-óptica de materiais 2Ds via nanoespectroscopia de infravermelho síncrotron
• Beneficiário: Raul de Oliveira Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores