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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nano-Infrared Imaging of Primary Neurons

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Author(s):
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Freitas, Raul O. [1] ; Cernescu, Adrian [2] ; Engdahl, Anders [3] ; Paulus, Agnes [3, 4] ; Levandoski, Joao E. [1] ; Martinsson, Isak [5] ; Hebisch, Elke [6] ; Sandt, Christophe [7] ; Gouras, Gunnar Keppler [5] ; Prinz, Christelle N. [6] ; Deierborg, Tomas ; Borondics, Ferenc [7] ; Klementieva, Oxana [3]
Total Authors: 13
Affiliation:
[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil
[2] Attocube Syst AG, Eglfinger Weg 2, D-85540 Munich - Germany
[3] Lund Univ, Dept Expt Med Sci, Med Microspectroscopy, S-22180 Lund - Sweden
[4] Lund Univ, Dept Expt Med Sci, Expt Neuroinflammat Lab, S-22180 Lund - Sweden
[5] Lund Univ, Dept Expt Med Sci, Expt Dementia Res, S-22180 Lund - Sweden
[6] Lund Univ, Div Solid State Phys & NanoLund, S-22100 Lund - Sweden
[7] Synchrotron SOLEIL, F-91192 Gif Sur Yvette - France
Total Affiliations: 7
Document type: Journal article
Source: CELLS; v. 10, n. 10 OCT 2021.
Web of Science Citations: 0
Abstract

Alzheimer's disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-beta (A beta) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, A beta proteins undergo structural changes to form beta-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid beta-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM) to study amyloid structures at the nanoscale, in individual neurons. Specifically, we show that in well-validated systems, s-SNOM can detect amyloid beta-sheet structures with nanometer spatial resolution in individual neurons. This is a proof-of-concept study to demonstrate that s-SNOM can be used to detect A beta-sheet structures on cell surfaces at the nanoscale. Furthermore, this study is intended to raise neurobiologists' awareness of the potential of s-SNOM as a tool for analyzing amyloid beta-sheet structures at the nanoscale in neurons without the need for immunolabeling.</p> (AU)

FAPESP's process: 19/14017-9 - From excitons waves to THz-phonons: 2Ds materials nano-optics via synchrotron infrared nanospectroscopy
Grantee:Raul de Oliveira Freitas
Support Opportunities: Research Grants - Young Investigators Grants