| Texto completo | |
| Autor(es): Mostrar menos - |
Barbosa, Eder Alves
[1, 2]
;
Placido, Alexandra
[3, 4]
;
Moreira, Daniel C.
[5]
;
Albuquerque, Lucas
[6]
;
Dematei, Anderson
[5, 7]
;
Silva-Carvalho, Amandda E.
[8]
;
Cabral, Wanessa F.
[5]
;
Bao, Sonia N.
[9]
;
Saldanha-Araujo, Felipe
[8]
;
Kuckelhaus, Selma A. S.
[5]
;
Borges, Tatiana K.
[6]
;
Portugal, Camila C.
[10, 11]
;
Socodato, Renato
[10, 11]
;
Teixeira, Catia
[3]
;
Lima, Filipe Camargo D. A.
[12]
;
Batagin-Neto, Augusto
[13]
;
Sebben, Antonio
[14]
;
Eaton, Peter
[3, 15]
;
Gomes, Paula
[3]
;
Brand, Guilherme D.
[2]
;
Relvas, Joao B.
[10, 11]
;
Kato, Massuo J.
[16]
;
Leite, Jose Roberto S. A.
[5, 4]
Número total de Autores: 23
|
| Afiliação do(s) autor(es): Mostrar menos - | [1] EMBRAPA Recursos Genet & Biotecnol, Lab Espectrometria Massa, Brasilia, DF - Brazil
[2] Univ Brasilia, Inst Quim, Lab Sintese & Anal Biomol, Brasilia, DF - Brazil
[3] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, LAQV REQUIMTE, Porto - Portugal
[4] Bioprospectum Lda, UPTEC, Porto - Portugal
[5] Univ Brasilia, Nucleo Pesquisa Morfol & Imunol Aplicada NuPMIA, Fac Med, Brasilia, DF - Brazil
[6] Univ Brasilia, Lab Imunol Celular, Fac Med, NuPMIA, Brasilia, DF - Brazil
[7] Univ Brasilia, Fac Med, Nucleo Med Trop, Programa Posgrad Med Trop, Brasilia, DF - Brazil
[8] Univ Brasilia, Fac Ciencias Saude, Lab Hematol & Celulas Tronco, Brasilia, DF - Brazil
[9] Univ Brasilia, Lab Microscopia & Microanal, Inst Ciencias Biol, Brasilia, DF - Brazil
[10] Univ Porto, Inst Biol Mol & Celular, Porto - Portugal
[11] Univ Porto, Glial Cell Biol Lab, Inst Invest & Inovacao Saude, Porto - Portugal
[12] Inst Fed Educ Ciencia & Tecnol Sao Paulo, Matao - Brazil
[13] Sao Paulo State Univ UNESP, Campus Itapeva, Itapeva - Brazil
[14] Univ Brasilia, Inst Ciencias Biol, Brasilia, DF - Brazil
[15] Univ Lincoln, Joseph Banks Labs, Sch Chem, The Bridge, Lincoln - England
[16] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo, SP - Brazil
Número total de Afiliações: 16
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES; v. 288, n. 1962 NOV 10 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ({[}M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O-2 levels and UV exposure on land compared with aquatic environments. (AU) | |
| Processo FAPESP: | 18/07999-7 - VIDA-FROG: valorização de moléculas isoladas de anfíbios portugueses |
| Beneficiário: | Massuo Jorge Kato |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |