Current Status of Endolysin-Based Treatments against Gram-Negative Bacteria

Autor(es)

Pardini Gontijo, Marco Tulio ^[1] ; Jorge, Genesy Perez ^[1] ; Brocchi, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, UNICAMP, Rua Monteiro Lobato 255, BR-13083862 Campinas - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: ANTIBIOTICS-BASEL; v. 10, n. 10 OCT 2021.
• Citações Web of Science: 0

Resumo

The prevalence of multidrug-resistant Gram-negative bacteria is a public health concern. Bacteriophages and bacteriophage-derived lytic enzymes have been studied in response to the emergence of multidrug-resistant bacteria. The availability of tRNAs and endolysin toxicity during recombinant protein expression is circumvented by codon optimization and lower expression levels using inducible pET-type plasmids and controlled cultivation conditions, respectively. The use of polyhistidine tags facilitates endolysin purification and alters antimicrobial activity. Outer membrane permeabilizers, such as organic acids, act synergistically with endolysins, but some endolysins permeate the outer membrane of Gram-negative bacteria per se. However, the outer membrane permeation mechanisms of endolysins remain unclear. Other strategies, such as the co-administration of endolysins with polymyxins, silver nanoparticles, and liposomes confer additional outer membrane permeation. Engineered endolysins comprising domains for outer membrane permeation is also a strategy used to overcome the current challenges on the control of multidrug-resistant Gram-negative bacteria. Metagenomics is a new strategy for screening endolysins with interesting antimicrobial properties from uncultured phage genomes. Here, we review the current state of the art on the heterologous expression of endolysin, showing the potential of bacteriophage endolysins in controlling bacterial infections.</p> (AU)

• Processo FAPESP: 20/01535-9 - Genômica comparativa e atividade antimicrobiana de endolisinas fágicas recombinantes contra bactérias gram-negativas multirresistentes
• Beneficiário: Marco Túlio Pardini Gontijo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/10051-2 - Potencial biotecnológico e caracterização fenotípica e molecular de mutantes de Salmonella enterica
• Beneficiário: Marcelo Brocchi
• Modalidade de apoio: Auxílio à Pesquisa - Regular