Lactobacillus casei reduces the extracellular matrix components of fluconazole-susceptible Candida albicans biofilms

Autor(es)

Panariello, Beatriz H. D. ^{[1, 2]} ; Klein, Marlise Inez ^[1] ; Dias, Luana Mendonca ^[1] ; Bellini, Amanda ^[1] ; Costa, Vitoria Bonan ^{[1, 3]} ; Barbugli, Paula Aboud ^[1] ; Pavarina, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, UNESP, Araraquara, SP - Brazil

^[2] Indiana Univ, Dept Cariol Operat Dent & Dent Publ Hlth, Sch Dent, Indianapolis, IN 46204 - USA

^[3] Sao Paulo State Univ, Sch Dent, Dept Surg & Diag, UNESP, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 37, n. 9-10 NOV 2021.
• Citações Web of Science: 0

Resumo

Fluconazole-sensitive (CaS) and -resistant (CaR) C. albicans were grown as single-species and dual-species biofilms with Lactobacillus casei (Lc) and Lactobacillus rhamnosus (Lr). Single-species Lc and Lr were also evaluated. Biofilm analysis included viable plate counts, the extracellular matrix components, biomass, and structural organization. Lc reduced the viability of CaS, water-soluble polysaccharides, and eDNA in CaS + Lc biofilm. Lc biofilm presented more eDNA than CaS. The total biomass of CaS + Lc biofilm was higher than the single-species biofilms. The viability of Lc and Lr was reduced by CaR dual-species biofilms. The total and insoluble biomass in CaS + Lr was higher than in single-species CaS biofilms. Lc hindered the growth of CaS, and their association hampered matrix components linked to the structural integrity of the biofilm. These findings allow understanding of how the implementation of probiotics influences the growth of C. albicans biofilms and thereby helps with the development of novel approaches to control these biofilms. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/18804-1 - Matriz extracelular de biofilmes de Candida albicans e Candida glabrata susceptíveis e resistentes ao fluconazol: Caracterização, ação de enzimas hidrolíticas e eficácia da associação das enzimas à Terapia Fotodinâmica.
• Beneficiário: Beatriz Helena Dias Panariello
• Modalidade de apoio: Bolsas no Brasil - Doutorado