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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lactobacillus casei reduces the extracellular matrix components of fluconazole-susceptible Candida albicans biofilms

Full text
Author(s):
Panariello, Beatriz H. D. [1, 2] ; Klein, Marlise Inez [1] ; Dias, Luana Mendonca [1] ; Bellini, Amanda [1] ; Costa, Vitoria Bonan [1, 3] ; Barbugli, Paula Aboud [1] ; Pavarina, Ana Claudia [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, UNESP, Araraquara, SP - Brazil
[2] Indiana Univ, Dept Cariol Operat Dent & Dent Publ Hlth, Sch Dent, Indianapolis, IN 46204 - USA
[3] Sao Paulo State Univ, Sch Dent, Dept Surg & Diag, UNESP, Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOFOULING; v. 37, n. 9-10 NOV 2021.
Web of Science Citations: 0
Abstract

Fluconazole-sensitive (CaS) and -resistant (CaR) C. albicans were grown as single-species and dual-species biofilms with Lactobacillus casei (Lc) and Lactobacillus rhamnosus (Lr). Single-species Lc and Lr were also evaluated. Biofilm analysis included viable plate counts, the extracellular matrix components, biomass, and structural organization. Lc reduced the viability of CaS, water-soluble polysaccharides, and eDNA in CaS + Lc biofilm. Lc biofilm presented more eDNA than CaS. The total biomass of CaS + Lc biofilm was higher than the single-species biofilms. The viability of Lc and Lr was reduced by CaR dual-species biofilms. The total and insoluble biomass in CaS + Lr was higher than in single-species CaS biofilms. Lc hindered the growth of CaS, and their association hampered matrix components linked to the structural integrity of the biofilm. These findings allow understanding of how the implementation of probiotics influences the growth of C. albicans biofilms and thereby helps with the development of novel approaches to control these biofilms. (AU)

FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/18804-1 - Extracellular matrix of susceptible and fluconazole-resistant Candida albicans and Candida glabrata biofilms: characterization, action of hydrolytic enzymes and efficacy of the combination of enzymes to Photodynamic Therapy.
Grantee:Beatriz Helena Dias Panariello
Support Opportunities: Scholarships in Brazil - Doctorate