Toxocara DNA amplification in serum and tissue samples in BALB/c mice

Autor(es)

Rodrigues e Fonseca, Gabriela ^{[1, 2]} ; de Melo, Gessica Baptista ^{[1, 2]} ; de Paula, Fabiana Martins ^{[1, 2]} ; Malta, Fernanda Mello ^{[3, 4]} ; Borges Gryschek, Ronaldo Cesar ^{[1, 2]} ; Zevallos Lescano, Susana Angelica ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Invest Med Imunopatol Esquistossomose LIM 06, Hosp Clin, Fac Med, Av Dr Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Med Trop, Fac Med, Ave Dr Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Lab Gastroenterol Clin Expt LIM 07, Hosp Clin, Fac Med, Av Dr Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil

^[4] Hosp Israelita Albert Einstein, Av Albert Einstein 627-701, BR-05652900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Biochemical Parasitology; v. 246, NOV 2021.
• Citações Web of Science: 0

Resumo

Toxocariasis is still a neglected parasitic disease worldwide and much about its biology and diagnosis has yet to be understood. The migration of third stage larvae via bloodstream suggests a potential use of molecular tools in diagnosis as well to deepen the knowledge about its migration behaviors. Conventional PCR was applied in serum and tissue samples from BALB/c mice infected with 5 and 500 embryonated eggs. Blood samples were collected at 15, 30, 60, 90 and 120 days post-infection. Organs were excised at 170 days post infection. There was no DNA amplification in serum samples in any group or day post-infection; contrarily, tissue samples showed DNA amplification. These results also support a continuous larval migration after and/or simultaneously with the neurotropic-myotropic phase. Thus, molecular tools might be useful as a differential diagnosis method, but do not replace immunodiagnostics techniques. (AU)

• Processo FAPESP: 14/07345-6 - PCR para detecção de DNA de Toxocara canis em amostras de soro de camundongos experimentalmente infectados, comparação com técnicas imunológicas
• Beneficiário: Susana Angélica Zevallos Lescano
• Modalidade de apoio: Auxílio à Pesquisa - Regular