Toxocara DNA amplification in serum and tissue samples in BALB/c mice

Author(s)

Rodrigues e Fonseca, Gabriela ^{[1, 2]} ; de Melo, Gessica Baptista ^{[1, 2]} ; de Paula, Fabiana Martins ^{[1, 2]} ; Malta, Fernanda Mello ^{[3, 4]} ; Borges Gryschek, Ronaldo Cesar ^{[1, 2]} ; Zevallos Lescano, Susana Angelica ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Lab Invest Med Imunopatol Esquistossomose LIM 06, Hosp Clin, Fac Med, Av Dr Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Med Trop, Fac Med, Ave Dr Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Lab Gastroenterol Clin Expt LIM 07, Hosp Clin, Fac Med, Av Dr Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil

^[4] Hosp Israelita Albert Einstein, Av Albert Einstein 627-701, BR-05652900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Molecular and Biochemical Parasitology; v. 246, NOV 2021.
• Web of Science Citations: 0

Abstract

Toxocariasis is still a neglected parasitic disease worldwide and much about its biology and diagnosis has yet to be understood. The migration of third stage larvae via bloodstream suggests a potential use of molecular tools in diagnosis as well to deepen the knowledge about its migration behaviors. Conventional PCR was applied in serum and tissue samples from BALB/c mice infected with 5 and 500 embryonated eggs. Blood samples were collected at 15, 30, 60, 90 and 120 days post-infection. Organs were excised at 170 days post infection. There was no DNA amplification in serum samples in any group or day post-infection; contrarily, tissue samples showed DNA amplification. These results also support a continuous larval migration after and/or simultaneously with the neurotropic-myotropic phase. Thus, molecular tools might be useful as a differential diagnosis method, but do not replace immunodiagnostics techniques. (AU)

• FAPESP's process: 14/07345-6 - Use of a polymerase chain reaction technique for Toxocara canis DNA detection in sera of experimentally infected mice
• Grantee: Susana Angélica Zevallos Lescano
• Support Opportunities: Regular Research Grants