Experimental effects of cadmium on physiological response of Callinectes danae (Crustacea, Portunidae) from environments with different levels of Cd contamination

Autor(es)

Ortega, Priscila ^[1] ; Vitorino, Hector Aguilar ^{[2, 3]} ; Green, Shadaesha ^[4] ; Zanotto, Flavia P. ^[5] ; Chung, J. Sook ^[4] ; Moreira, Renata G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias IB, Lab Metab & Reprod Organismos Aquat LAMEROA, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias IB, Lab Quim Bioinorgan & Metalofarmacos LAQBAM, Sao Paulo - Brazil

^[3] Natl Univ Engn, Fac Sci, BIOMET Res Grp, Av Tupac Amaru 210, Lima 15333 - Peru

^[4] Univ Maryland Ctr Environm Sci UMCES, Inst Marine & Environm Technol IMET, Baltimore, MD - USA

^[5] Univ Sao Paulo, Inst Biociencias IB, Lab Biol Celular Invertebrados Marinhos LabCel, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 251, n. SI JAN 2022.
• Citações Web of Science: 0

Resumo

Cadmium (Cd) can adversely affect aquatic life, altering reproductive and molting processes in crustaceans. The objective of this study was to evaluate the influence of Cd on reproduction and molting in the crab Callinectes danae. Adult females were obtained from environments with different levels of pollution: low (LC), medium (MC), and high contaminated (HC) areas. Animals from LC, MC, and HC areas were exposed to 0, 0.5, and 2 mg L-1 of CdCl2 for 3 h. Cd bioaccumulation, oxidative stress (evaluated by antioxidant enzymes activity), and lipid peroxidation (LPX) were analyzed in mature ovaries (stage II), gills, and hepatopancreas. The expression levels of crustacean hyperglycemic hormone (CHH) and molt-inhibiting hormone (MIH) genes were quantified in the eyestalks, while 17 beta-estradiol (E2) and melatonin concentration were measured in the hemolymph. Cd bioaccumulated mainly in the hepatopancreas and gills, with increased E2, LPX, and antioxidant enzymes in HC compared to the LC region. Decreased CHH and MIH transcripts were observed in the animals from HC regions compared to LC and MC areas. Physiological differences were recorded, especially for bioaccumulation, oxidative stress, and hormone levels, in animals sampled in HC areas compared to LC and MC regions. In conclusion, the physiological damage triggered by Cd could be reduced due to higher levels of melatonin and antioxidant enzymes in HC areas. (AU)

• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 17/12439-8 - Cádmio e os processos fisiológicos de reprodução e muda em Callinectes danae: comparação entre um contínuo de ambientes com diferentes níveis de poluição
• Beneficiário: Priscila Ortega
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/11639-6 - Interação entre cádmio e a fisiologia reprodutiva em Callinectes danae de ambientes com diferentes níveis de poluição
• Beneficiário: Priscila Ortega
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado