Malva sylvestris derivatives as inhibitors of HIV-1 BaL infection

Autor(es)

Benso, Bruna ^{[1, 2, 3]} ; Rosalen, Pedro Luiz ^[1] ; Pasetto, Silvana ^{[4, 5]} ; Salome Marquezin, Maria Carolina ^[1] ; Freitas-Blanco, Veronica ^[1] ; Murata, Ramiro Mendonca ^{[4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[2] Pontificia Univ Catolica Chile, Fac Med, Sch Dent, Santiago, RM - Chile

^[3] Pontificia Univ Catolica Chile, Fac Med, Pharmacol & Toxicol, Santiago, RM - Chile

^[4] East Carolina Univ, Dept Fdn Sci, Sch Dent Med, Greenville, NC 27858 - USA

^[5] East Carolina Univ, Dept Microbiol & Immunol, Brody Sch Med, Greenville, NC 27858 - USA

• Tipo de documento: Artigo Científico
• Fonte: NATURAL PRODUCT RESEARCH; v. 35, n. 6, p. 1064-1069, MAR 19 2021.
• Citações Web of Science: 3

Resumo

The emphasis of the present study is to evaluate a natural product and the potential microbicide activity using a dual chamber infection method. Malva sylvestris extracts and fractions were screened for anti-HIV activity by measuring the virus-antibody neutralization. Plant extracts with strong antiviral activity working in nanomolar or picomolar range can be used to enhance the activity of synthetic compounds and work as anti-HIV agents. The aqueous fraction (AF) of M. sylvestris demonstrated antiviral activity in a model with epithelial and blood cell lines. The AF showed an effective antiviral potential on the TZM-bl cells with reduction scores higher than 60% of infectivity. Quantification of p24 in the supernatant of the co-culture model demonstrated a reduction in the number of viral particles after AF treatment (p < 0.05). Cytokines were quantified and all signaling inflammatory markers; IL1-alpha, IL-beta, IL-6, IL-8 and GM-CSF (p < 0.05) were modulated by positive control and AF treatments. In particular, IL-6 had lower levels of expression in Malva groups when compared to the Zidovudine positive control group. Natural occurring derivatives of M. sylvestris demonstrated to work inhibiting reverse transcriptase enzyme action. M. sylvestris contains highly potential anti-HIV-1 BaL components and may be considered a potential source for new formulations in the development of topical microbicides. (AU)

• Processo FAPESP: 11/23980-5 - Avaliação do potencial antimicrobiano, antiinflamatório, remodelação óssea, antioxidante e antineoplásico da Malva sylvestris.
• Beneficiário: Bruna Benso
• Modalidade de apoio: Bolsas no Brasil - Doutorado