Controlled spatial organization of bacterial growth reveals key role of cell filamentation preceding Xylella fastidiosa biofilm formation

Autor(es)

Anbumani, Silambarasan ^[1] ; da Silva, Aldeliane M. ^[1] ; Carvalho, Isis G. B. ^[2] ; Fischer, Eduarda Regina ^[2] ; de Souza e Silva, Mariana ^[2] ; von Zuben, Antonio Augusto G. ^[1] ; Carvalho, Hernandes F. ^[3] ; de Souza, Alessandra A. ^[2] ; Janissen, Richard ^[4] ; Cotta, Monica A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, BR-13083859 Campinas, SP - Brazil

^[2] Citrus Ctr APTA Sylvio Moreira Agron Inst Campina, BR-13490970 Cordeiropolis, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil

^[4] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: NPJ BIOFILMS AND MICROBIOMES; v. 7, n. 1 DEC 7 2021.
• Citações Web of Science: 0

Resumo

The morphological plasticity of bacteria to form filamentous cells commonly represents an adaptive strategy induced by stresses. In contrast, for diverse human and plant pathogens, filamentous cells have been recently observed during biofilm formation, but their functions and triggering mechanisms remain unclear. To experimentally identify the underlying function and hypothesized cell communication triggers of such cell morphogenesis, spatially controlled cell patterning is pivotal. Here, we demonstrate highly selective cell adhesion of the biofilm-forming phytopathogen Xylella fastidiosa to gold-patterned SiO2 substrates with well-defined geometries and dimensions. The consequent control of both cell density and distances between cell clusters demonstrated that filamentous cell formation depends on cell cluster density, and their ability to interconnect neighboring cell clusters is distance-dependent. This process allows the creation of large interconnected cell clusters that form the structural framework for macroscale biofilms. The addition of diffusible signaling molecules from supernatant extracts provides evidence that cell filamentation is induced by quorum sensing. These findings and our innovative platform could facilitate therapeutic developments targeting biofilm formation mechanisms of X. fastidiosa and other pathogens. (AU)

• Processo FAPESP: 13/10957-0 - Interação Xylella fastidiosa-inseto vetor-planta hospedeira e abordagens para o controle da clorose variegada dos citros e cancro cítrico
• Beneficiário: Alessandra Alves de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/07616-3 - Novos catalisadores e controle de direcionalidade em nanofios semicondutores do tipo III-V
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático